Principle of Cre-DIO system - Genemedi

Figure 6. Principle of Cre-DIO (double-floxed inverse open reading frame) system. Without Cre, the GOI and reporter gene are inverted and silenced. In ... PrincipleofCre-DIOsystem ViewKnowledgeBase-Cre-loxpsystemandViralvector(AAVandadenovirus)-basedCretools(AAV-CreandAd-Cre)>> Productlist:Cre/loxPtoolsinAAVvector(AAV-Cre),adenoviralvector(Ad-Cre)andlentiviralvector(Lv-Cre) CatNo. Pre-madeCre Viralvector Protomer PromoterTissueSpecificity PromoterTissue Specificitycharacteristics GMV-AdCre-01Ad-CMV-CreAdenovirusCMVTotalTotal GMV-AdCre-02Ad-CMV-Cre-GFPCMVTotalTotal GMV-AAV9Cre-01AAV9-CMV-CreAAV GeneMedioffermultipleserotypesofAAV-CreloxPsystemforyouroption: ·AAV1-Cre ·AAV2-Cre ·AAV2variant(Y444F)-Cre ·AAV2variant(Y272F,Y444F,Y500F,Y730F)-Cre ·AAV2variant(Y444F,Y730F,Y500F,Y272F,Y704F,Y252F)-Cre ·AAV2variant(AAV2.7m8)-Cre ·AAV5-Cre ·AAV6-Cre ·AAV8-Cre ·AAV8-1m-Cre ·AAV8-2m-Cre ·AAV8variant(Y733F,Y447F,Y275)-Cre ·AAV9-Cre ·AAV-Rh.10-Cre ·AAV-DJ-Cre ·AAV-DJ/8-Cre ·AAV-Retro(Retrograde)-Cre ·AAV9-PHP.B-Cre ·AAV9-PHP.A-Cre ·AAV9-PHP.eB-Cre ·AAV9-PHP.S-Cre CMVTotalTotal GMV-AAV9Cre-02AAV9-CMV-Cre-ZsGreenCMVTotalTotal GMV-AAV9Cre-03AAV9-CAG-CreCAGTotalTotal GMV-AAV9Cre-04AAV9-CAG-Cre-ZsGreenCAGTotalTotal GMV-AAV9Cre-05AAV9-Syn-Cre-EGFPsynapsin(SYN) NeuroNeuron-specificpromoter GMV-AAV9Cre-06AAV9-Syn-Cresynapsin(SYN) NeuroNeuron-specificpromoter GMV-AAV9Cre-07AAV9-CaMKII-Cre-EGFPCaMKIINeuroForebrainglutamateneuron-specificpromoter GMV-AAV9Cre-08AAV9-CaMKII-CreCaMKIINeuroForebrainglutamateneuron-specificpromoter GMV-AAV9Cre-09AAV9-GFAP-Cre-EGFPGFAPNeuroAstrocytespecificpromoter GMV-AAV9Cre-10AAV9-GFAP-CreGFAPNeuroAstrocytespecificpromoter GMV-AAV9Cre-11AAV9-cTNT-CrecTnTheartCardiomyocytespecificpromoter GMV-AAV9Cre-12AAV9-cTNT-Cre-EGFPcTnTheartCardiomyocytespecificpromoter GMV-AAV9Cre-13AAV9-TBG-CreTBGliverHepaticspecificpromoter GMV-AAV9Cre-14AAV9-TBG-Cre-ZsGreenTBGliverHepaticspecificpromoter GMV-AAV9Cre-15AAV9-TIE-CreTIEendothelialEndothelial-specificpromoter GMV-AAV9Cre-16AAV9-cd68-Crecd68NeuroMicroglia-specificpromoter GMV-AAV9Cre-17AAV9-c-fos-Crec-fosNeuroExcitatoryneuronpromoter GMV-AAV9Cre-18AAV9-mecp2-Cremecp2NeuroShorterneuron-specificpromoter GMV-AAV9Cre-19AAV9-MHCK7-CreMHCK7muscleMusclespecificpromoter GMV-AAV9Cre-20AAV9-Rpe65-CreRpe65retinaRetinalspecificpromoter GMV-AAV9Cre-21AAV9-sm22a-Cresm22amuscleSmoothmusclespecificpromoter GMV-AAV9Cre-22AAV9-Spc5-12-CreSpc5-12muscleMyocytespecificpromoter GMV-AAV9Cre-23AAV9-Ta1-CreTalNeuroEarlyneuron-specificpromoter GMV-LvCre-01Lv-CMV-CreLentivirusCMVTotalTotal GMV-LvCre-02Lv-CMV-Cre-zsgreenCMVTotalTotal ForPricepleaseclickhereforinquiry.>> Abstract Cre-loxPsystemiswidelyusedinthefieldofbiosciences,especiallyinthegenerationofgeneticallyengineeredmice(knockoutoroverexpression),enablingresearcherstostudythefunctionofgeneofinterest(GOI).Todate,numeroussystems,derivedfromthebasicCre-loxPsystem,havebeendevelopedtopreciselycontrolgeneexpressionatdesiredtimeorcells.Here,webrieflyintroduceCre-loxPsystem,andgiveasummaryaboutitsapplications,especiallyviralvectors-mediatedCreexpression(suchasAAV-CreorAd-Cre)forinvitroorinvivostudies. PrincipleofCre-DIO AlthoughCre-loxPtooliswidespreadinbiologicalsciences,itisreallyrestrictivetoachievemorethanonerecombinationeventinacell.Therefore,numerousloxPmutantversionshavebeencreated,withthevariationfromtheunique8bpcoreasymmetricspacer"NNNTANNN",suchasloxN(GtATACcT),lox2272(GgATACtT),lox5171(ATGTGTaC),loxM2(AgaaAcca),loxM3(taaTACCA),loxM11(AGATAGAA),andlox511(GtATACAT).Theseloxvariantsonlyproceedrecombinationwiththesametypeofloxsites,withnoabilitytointeractwiththeothertypes.InCre-DIOsystem,therearetwopairsofloxsites(loxsite1andloxsite2)flankinginvertedGOIandreportergene.IntheabsenceofCre,theinvertedGOIandreporterareinactivated.UponadministrationofCre,thetwopairsofloxsiteswillundergorecombination,resultingintheexpressionofGOIandreportergene.Simultaneously,thecellswithrecombinationinteractionarelabeledbythereportergene(Fig.6).TheCre-DIOtoolutilizesinitiallyinvertedGOItotrulyinactivateGOI,andactivatesGOIafteraseriesofrecombinationinteractions.Comparedtothetraditionaloverexpressionstrategyviafloxedstop,thistechnologyismuchmoreeffectivetoavoidthetranscriptionalleakagewhenthestopcodonfailstototallysuppressthetranscriptionofGOI. Figure6.PrincipleofCre-DIO(double-floxedinverseopenreadingframe)system.WithoutCre,theGOIandreportergeneareinvertedandsilenced.InthepresenceofCre,first,thetransgenewillbereoriented,thenoneofthetwoloxsitesineachpairwillbeexcised.Finally,thetransgenecontainsonlyanincompatiblepairofloxsites(lox2-GOI-Reporter-lox1)thatarenolongerresponsivetoCre.Thus,theGOIandreportergenewillbeactivated.Loxsite1andloxsite2arevariantsofloxsites. Summary TheCre-loxPsystem,especiallyinducibletissue-specificknockoutandviralvector-mediatedCreexpression,hasbeenhighlyutilizedingeneticsandcellbiologyresearch.Nevertheless,itseemsthatCre-loxPsystemwillcontinuetobeprevalentincurrentandfutureresearchstudies.GeneMediisproficientinviralvectordevelopmentandofferskindsofviralvector-basedCretools,wecanprovidethebestservicesandproductsifrequired. References 1. SternbergN,HamiltonD.BacteriophageP1site-specificrecombination.I.RecombinationbetweenloxPsites.JMolBiol.1981;150:467-486. 2. 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