Cre-inducible Adeno-associated Virus: DIO-AAV - SignaGen

Cre-inducible Adeno-associated Virus: DIO-AAV. For cell type-specific targeting and to capitalize on the large number of Cre driver lines ... Skiptocontent Forcelltype-specifictargetingandtocapitalizeonthelargenumberofCredriverlineswiththeflexiblevirusinjection/fiberopticapproach,wehavedevelopedatoolwecallDIO-AAV(doublefloxedinverseorf)AAV. pAAV-Ef1a-DIO-hChR2(H134R)-EYFP-WPRE-pA [VectorMap] pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA [VectorMap] pAAV-Ef1a-DIO-eNpHR-EYFP-WPRE-pA [VectorMap] OpticalExcitation: Channelrhodopsin-2(ChR2) TherearetwoversionsoftheChR2sequence,onecontainingthewildtypesequenceandanothercontainingcondonsoptimizedformammalianexpression(hChR2).In-framefusionstomCherryorEYFPareavailabletomakevisualizationofChR2-expressingcellseasier.ChR2andXFParefusedviaaNotIsite.ThelinkerisGCGGCCGCC. ChR2-XFPsareavailableeitherinastandardmammalianexpressionvectorcontainingtheCMVpromoterorinlentiviralexpressionvectorsunderthecontroloftheubiquitousEF-1aortheneuron-specificCaMKIIaorhumanSynapsinIpromoters.Thestructureofthelentivirusisshownbelow. pcDNA3.1/hChR2-mCherry [VectorMap] pcDNA3.1/hChR2-EYFP [VectorMap] pcDNA3.1/hChR2(H134R)-EYFP [VectorMap] pAAV-CaMKIIa-hChR2(H134R)-EYFP [VectorMap] pAAV-CaMKIIa-hChR2(H134R)-mCherry [VectorMap] pAAV-hSyn-hChR2(H134R)-EYFP [VectorMap] pAAV-hSyn-hChR2(H134R)-mCherry [VectorMap] pAAV-hSyn-hChR2(H134R)-WPRE [VectorMap] pAAV-hSyn-hChR2(H134R)-p2A-WPRE [VectorMap] pAAV-mThy1-hChr2(H134R)-eYFP-WPRE [VectorMap] pAAV-hThy1-hChR2(H134R)-eYFP-WPRE [VectorMap] pAAV-GFAP-hChR2(H134R)-EYFP [VectorMap] pAAV-GFAP-hChR2(H134R)-mCherry [VectorMap] pAAV-EF1a-hChR2(H134R)-EYFP [VectorMap] pLenti-EF1a-hChR2-EYFP-WPRE(a.k.a.pLECYT) [VectorMap] pLenti-EF1a-hChR2(H134R)-EYFP-WPRE(a.k.a.pLECYT) [VectorMap] pLenti-CaMKIIa-hChR2-mCherry-WPRE [VectorMap] pLenti-CaMKIIa-hChR2-EYFP-WPRE [VectorMap] pLenti-Hcrt-hChR2(H134R)-EYFP [VectorMap] pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE [VectorMap] pLenti-mThy1-hChR2(H134R)-eYFP [VectorMap] pLenti-hThy1-hChR2(H134R)-eYFP [VectorMap] Adeno-associatedVirus(AAV)|signagen |October9,2015 LeaveaReplyCancelreplyYouremailaddresswillnotbepublished.Requiredfieldsaremarked* GCaMP6calciumindicators SignaGen’sAd.MAX™AdenovirusExpressionSystemforMaximumAdenovirusProduction Searchfor: Search RecentPosts LentivirusFormulationBuffer A195BufferforAdenovirusFormulationandLongTermStorage DNA/RNAnon-specificendonuclease[Serratiamarcescens] DNA/RNAnon-specificendonuclease[Serratiamarcescens] DNA/RNAnon-specificendonuclease[Serratiamarcescens] StartingMaterialstoRunaddPCRforrAAVTitrationwithAnAutoDGDropletGeneratorandQX200DropletReader TipsandtricksforperformingddPCR LV-EF1a-GFP-PuroinBaboonEnvelopePseudotype,ReadytoPackage AdenovirusTypeCqPCRTitrationPrimersandProbe DensityandRefractiveIndexforCsCl CellCultureAntibioticSelectionGuide AAV5-RPhpe427-GFPandAAV5-RPhpe1289-GFPReadytoPackage DOS&DON’TSOFPLASMIDPURIFICATION AAV9-hSyn-hM3D(Gq)-mCherryandAAV9-hSyn-hM4D(Gi)-mCherryReadytoPackage MembraneInformation QuantificationofAAVparticlesandemptycapsidsbyopticaldensitymeasurement qPCRDeterminationofMycoplasmainAAVSamples AAV10VP1Sequence ValidatedTargetRegionagainstHumanHSD17B13Gene ValidatedTargetRegionforTLR4Gene MouseEGR1ValidatedTargetRegion HumanChATPromoter ValidatedTargetRegionagainstMouseG3BPGene TwoValidatedMouseSV2CTargetRegions ValidatedTargetRegionagainstMouseCacna1cGene LunaUniversalProbeOne-StepRT-qPCRforDetectionofCovid-19 RT-qPCRReactionConditionsforDetectionofCovid-19byFDAandCDC AAV8-GFAP0.7-emGFP-miRNA(mPTBP1)ReadytoPackage ValidatedTargetRegionagainstMousePTBP1Gene ResearchUseOnly2019-NovelCoronavirus(2019-nCoV,Covid-19)Real-timeRT-PCRPrimersandProbes Bmi-1 ValidatedtargetRegions ValidatedsiRNATargetingMouseTrpv1Gene ANovelAAVSerotypeB1forCNSTransduction NovelAAV-FAAV-SforNeuronalCortexTransduction Virususedingenetherapiesmayposecancerrisk,dogstudyhints WhydoIseeamplificationcurvesinmyNTCsamples? CategoriesCategories SelectCategory Adeno-associatedVirus(AAV) Adenovirus Lentivirus Miscellaneous Transfection TransgenicAnimals Archives Archives SelectMonth August2022 January2022 December2021 November2021 September2021 July2021 April2021 March2021 February2021 January2021 December2020 November2020 October2020 September2020 August2020 July2020 June2020 May2020 April2020 February2020 January2020 November2019 October2019 September2019 August2019 July2019 June2019 May2019 February2019 December2018 November2018 October2018 September2018 July2018 June2018 May2018 April2018 March2018 February2018 January2018 November2017 October2017 August2017 May2017 March2017 January2017 October2016 September2016 August2016 July2016 June2016 May2016 April2016 March2016 February2016 January2016 December2015 November2015 October2015 October2015 M T W T F S S  1234 567891011 12131415161718 19202122232425 262728293031       Nov»