In vitro Transcription for RNA Synthesis - NEB

In vitro synthesis of single-stranded RNA molecules is a widely used laboratory procedure that is critical to RNA research, as well as to RNA ... Home Applications RNAAnalysis InvitroTranscriptionforRNASynthesis Invitrosynthesisofsingle-strandedRNAmoleculesisawidelyusedlaboratoryprocedurethatiscriticaltoRNAresearch,aswellastoRNAbiopharmaceuticals.Thistechniqueisversatileinthatitallowstheresearchertotailorsynthesisandintroducemodificationstoproduceatranscript.DownstreamapplicationsincludebiochemicalandmolecularcharacterizationofRNAforRNA-proteininteractionsandstructuralanalyses,generationofRNAaptamers,synthesisoffunctionalmRNAsforexpression,andgenerationofsmallRNAsforalterationofgeneexpression(e.g.,guideRNAs,RNAi).Furthermore,theuseofinvitrosynthesizedRNAhasbeeninstrumentalinthedevelopmentofRNAvaccinesandCRISPR/Cas9genomeeditingtools,generationofpluripotentstemcells,aswellasdevelopmentofRNAamplification-baseddiagnostics. High-yieldrobustIVTreactionsrequireoptimizationofeachreactioncomponent.NEBofferssix invitroRNAsynthesiskits,allofwhichhavebeenoptimizedtogeneratereproducibleyieldsofqualityRNA.Inaddition,individualcomponentscanalsobepurchasedforIVTandmRNAcapping. Rio,D.C.,etal.RNA:ALaboratoryManual.ColdSpringHarbor:ColdSpringHarbor LaboratoryPress,2011,205-220. Cooper,GeofferyM.TheCell:AMolecularApproach.4thed.WashingtonD.C.:ASMPress,2007.262-299. JaniB,FuchsR.JVisExp.2012Mar26(61).PMID:22473375 ChooseType: FAQs Protocols Tools&Resources Publications RecommendedHiScribeRNASynthesisKitsbyApplication RNAPolymeraseSelectionChart LegalInformation FAQs Protocols Tools&Resources Publications RecommendedHiScribeRNASynthesisKitsbyApplication RNAPolymeraseSelectionChart LegalInformation FAQsforInvitroTranscriptionforRNASynthesis CanNEBprovideIVTcomponentsinbulk? ProtocolsforInvitroTranscriptionforRNASynthesis CappedRNASynthesis(E2040) CappingProtocol(M2080) DNATemplatePreparation(E2040) EvaluationofReactionProducts(E2040) HighSpecificActivityRadiolabeledRNAProbeSynthesis(E2040) PurificationofSynthesizedRNA(E2040) RNASynthesiswithModifiedNucleotides(E2040) SP6InVitroTranscriptionReactionProtocol(M0207) StandardRNASynthesis(E2040) ProtocolforDephosphorylationof5´-endsofDNAusingAntarcticPhosphatase(NEB#M0289) CappedRNASynthesis(E2050) EvaluationofReactionProducts(E2050) PurificationofSynthesizedRNA(E2050) RNASynthesiswithModifiedNucleotides(E2050) StandardRNASynthesis(E2050) ProtocolforAvoidingRnaseContaminationusingMurineRnaseInhibitor(M0314) ProtocolforAvoidingRnaseContaminationusingRnaseInhibitor,HumanPlacenta(M0307) Poly(A)TailingofRNAusingE.coliPoly(A)Polymerase(NEB#M0276) EvaluationofReactionProducts(E2060) mRNAPurification(E2060) mRNAPurification(E2065) mRNASynthesiswithModifiedNucleotides(E2060) mRNASynthesiswithModifiedNucleotides(E2065) StandardmRNASynthesis(E2060) StandardmRNASynthesis(E2065) EvaluationofReactionProducts(E2065) sgRNASynthesisUsingtheHiScribe™QuickT7HighYieldRNASynthesisKit(NEB#E2050) EnGen®sgRNASynthesisKit,S.pyogenesProtocol(E3322) PurificationofSynthesizedRNA(E2070) RNASynthesisProtocols(E2070) ProtocolforCo-transcriptionalcappingusingCleanCap®ReagentAGfromTriLinkandHiScribe™T7HighYieldRNASynthesisKitfromNewEnglandBiolabs® StandardRNASynthesisProtocolusingtheHiScribeT7mRNAKitwithCleanCapReagentAG(NEB#E2080) mRNASynthesisProtocolwithModifiedNucleotidesusingtheHiScribeT7mRNAKitwithCleanCapReagentAG(NEB#E2080) Tools&Resources FeatureArticles MindyourcapsandPolyAtails UnderstandoptionsforselectingreagentsandtheextentofreagentinfluenceonsynthesizedmRNAcapfunctionality. Brochures RNASynthesisBrochure RNATechnicalGuide UsageGuidelines AvoidingRibonucleaseContamination PublicationsrelatedtoInvitroTranscriptionforRNASynthesis Wu,M.Z.,Asahara,H.,Tzertzinis,G.,Roy,B. (2020) SynthesisoflowimmunogenicityRNAwithhigh-temperatureinvitrotranscriptionRNA; PubMedID:31900329 Eyler,D.E.,Franco,M.K.,Batool,Z.,Wu,M.Z.,Dubuke,M.L.,Dobosz-Bartoszek,M.,Jones,J.D.,Polikanov,Y.S.,Roy,B.,Koutmou,K.S (2019) PseudouridinylatioofmRNAcodingsequencesalterstranslationProcNatlAcadSciUSA; 116(46),23068-23074..PubMedID:31672910, DOI:10.1073/pnas.1821754116 Potapov,V.,Fu,X.,Dai,N.,Correa,I.R.,Jr.,Tanner,N.A.,Ong,J.L (2018) BasemodificatonsaffectingRNApolymeraseandreversetranscriptasefidelityNucleicAcidsRes; 46(11):5753-5763,PubMedID:29750267 RecommendedHiScribeRNASynthesisKitsbyApplication RNAPolymeraseSelectionChart NEBoffersRNApolymerasesthatcanbeusedforinvitrosynthesisofRNAforawidevarietyofdownstreamapplications.Additionalpolymerasesareofferedforthegenerationofuntemplatedhomoribopolymerictailsforreversetranscriptionorlabeling. LegalInformation Thisproductiscoveredbyoneormorepatents,trademarksand/orcopyrightsownedorcontrolledbyNewEnglandBiolabs,Inc(NEB). WhileNEBdevelopsandvalidatesitsproductsforvariousapplications,theuseofthisproductmayrequirethebuyertoobtainadditionalthirdpartyintellectualpropertyrightsforcertainapplications. Formoreinformationaboutcommercialrights,pleaseemailusat [email protected]. Thisproductisintendedforresearchpurposesonly.Thisproductisnotintendedtobeusedfortherapeuticordiagnosticpurposesinhumansoranimals. Videos + InVitroTranscriptionandCappingofGaussiaLuciferasemRNAFollowedbyHeLaCellTransfection ThismethoddescribeshighyieldinvitrosynthesisofbothcappedanduncappedmRNAfromalinearizedplasmidcontainingtheGaussialuciferase(GLuc)gene.  FeaturedProducts HiScribe™T7mRNAKitwithCleanCap®ReagentAG HiScribe™T7ARCAmRNAKit(withtailing) HiScribe™T7ARCAmRNAKit HiScribe™ T7QuickHighYieldRNASynthesisKit HiScribe™T7HighYieldRNASynthesisKit HiScribe™SP6RNASynthesisKit mRNACap2'-O-Methyltransferase VacciniaCappingSystem FaustovirusCappingEnzyme EnGen®sgRNASynthesisKit,S.pyogenes RNaseInhibitor,Murine Chooseyourcountry NorthAmerica Canada UnitedStates Europe France Germany UnitedKingdom Asia-Pacific Australia China Japan NewZealand Singapore Ifyoudon'tseeyourcountryabove,pleasevisitour internationalsite SessionExpired Youhavebeenidleformorethan20minutes,foryoursecurityyouhavebeenloggedout.Pleasesignbackintocontinueyoursession. 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