In Vitro Transcription | RNA Synthesis Kits | RNA Production

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FindSalesContact About AboutPromega CompanyInformation Newsroom CorporateCampusMap JoinOurTeam CareerOpportunities Sustainability CorporateResponsibility CommunityTouch PlanetAware PeopleCare CorporateMind ProductReach ContactUs CustomerSupport YourCart CurrentItems0 ViewCart Checkout Addmultipleproducts Home Products RNAAnalysis InVitroTranscription InvitrotranscriptionproductsincludeRNAsynthesissystems,nuclearextracts,ribonucleotides,cloningvectorsandsupportingproductsfortranscription,primerextensionandgeneexpressionstudies. Riboprobe®Systemsareusedtosynthesizehigh-specific-activitysingle-strandedRNAprobesormicrogramquantitiesofdefinedRNAtranscriptsfromclonedDNAinserts.  TheRiboMAX™RNAProductionSystemsgeneratelargequantitiesofcappedoruncappedRNAtranscriptssuitableforinvitroorinvivotranslationreactions.TheT7RiboMAX™ExpressLarge-ScaleRNAProductionSystemisaninvitrotranscriptionsystemdesignedfortheconsistentproductionoflargeamountsofRNAinashortamountoftime.Milligramamountsofhigh-qualityRNAtranscriptsupto14kbhavebeengeneratedusingthiskit. LearnaboutInVitroTranscription ShopInVitroTranscriptionProducts 23 FilterBy Clearallfilters ProductType Buffers InVitroTranscriptionSystems NuclearExtract Nucleotides PurificationAccessories Vectors ShopallInVitroTranscription Sortby: Newest AlphabeticalA-Z AlphabeticalZ-A Viewmoreresults Showing23of23Products RNASynthesisKitComparison  CompareRiboprobe®,RiboMAX™andRiboMAX™ExpressSystemfeaturesandRNAyields.   Riboprobe®Systems RiboMAX™LargeScaleRNAProductionSystems T7RiboMAX™ExpressLargeScaleRNAProductionSystem T7RiboMAX™ExpressRNAiSystem Cat.# P1420,P1430,P1440 P1280,P1300 P1320 P1700 Time 1hour 2–4hours 30minutes 30minutes RNAYield (per1mlreaction) Low (100–250µg) Medium (2–5mg) High (5–8.5mg) ≥500µgsiRNA ≥2mgdsRNA Radiolabeling T3Promoter SP6Promoter T7Promoter PremixedrNTPs CompatiblewithModifiedNucleotides NT 1 NT NT=nottested. 1=Compatible,butrequirescustomorderoftranscriptionbufferwithoutnucleotides. InVitroTranscriptionResources Resource DevelopmentofmRNAmanufacturingforvaccinesandtherapeutics Resource Blog:CommonCausesofinvitroTranscriptionReactionFailure Resource TheManyUsesforInVitroTranscribedRNA AnIntroductiontoRNASynthesisMethods InvitroRNAsynthesismethodsusebacteriophageDNA-dependentRNApolymerasesuchasT7,T3orSP6RNApolymerasetosynthesizeRNAfromaDNAtemplate.ThetemplateDNAforinvitrotranscriptionreactionsincludesanRNApolymerasepromoterupstreamofthesequenceofinterest.ThecorrespondingRNApolymeraseisthenusedtoproducesyntheticRNAtranscriptsforuseashybridizationprobes,astemplatesforinvitrotranslationapplications,orinstructuralstudies(X-raycrystallographyandNMR).SynthesizedRNAtranscriptsarealsousedforstudyingcellularRNAfunctionalityinprocessessuchassplicing,RNAprocessing,intracellulartransport,viralinfectivityandtranslation. RelatedNucleicAcidAnalysisProductsandResources Categories NucleicAcidAnalysis CloningandDNAMarkers EpigeneticsResearchKitsandReagents NucleicAcidExtraction PCR RNAAnalysis Sequencing Vectors RelatedGroups DNAandRNAQuantitation PlasmidPurification RNAExtraction GuidesandSelectors SubcloningGuide DNAPurification Epigenetics PCRAmplification TopProducts RiboMAX™Large-ScaleRNAProductionSystems Riboprobe®Systems PureYield™PlasmidMiniprepSystem Let'sfindtheproductthatmeetsyourneeds. 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