MEGAscript™ T7 Transcription Kit

The MEGAscript™ T7 Kit contains in vitro transcription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately ... PopularApplications&TechniquesShopAllProductsServicesSupport Signin QuickOrder     Search ThermoFisherScientific SearchAll Search HomeShopAllProductsBiochemicalReagentsTranscriptionandTranslationReagentsMEGAscript™T7TranscriptionKitInvitrogen™MEGAscript™T7TranscriptionKitCatalognumber:AM1334Relatedapplications:InVitroTranscriptionTechnicalSupportCustomerServiceInvitrogen™MEGAscript™T7TranscriptionKitCatalognumber:AM1334Relatedapplications:InVitroTranscriptionTechnicalSupportCustomerServiceCatalogNumberAM1334UnitSize40reactionsPrice(SGD)RequestAQuoteCatalogNumberAMB13345UnitSize200reactionsPrice(SGD)RequestAQuoteCatalogNumberAM1333UnitSize25reactionsPrice(SGD)RequestAQuoteCatalogNumberUnitSizePrice(SGD)AM133440reactionsRequestAQuoteAMB13345200reactionsRequestAQuoteAM133325reactionsRequestAQuoteProductOverviewFiguresVideosDocumentsFAQCitations&ReferencesAdditionalInformationRecommendationsRecommendationsTheMEGAscript™T7Kitisanultra-highyieldinvitrotranscriptionkit.Thehighyieldsareachievedbymodifyingtypicaltranscriptionreactionconditionssothatveryhighnucleotideconcentrationscanbeeffectivelyused.TheT7EnzymeMixandthe10XReactionBufferarespecificallycalibratedforeachlotandRNApolymerase.UsingtheMEGAscript™T7KitTheMEGAscript™T7Kitcontainsinvitrotranscriptionreactioncomponentsandacontroltemplate.Thekitwillyieldatotalof3–5mgofRNA(approximately100µgofRNAormoreperreaction)fromthecontroltemplatesuppliedwiththekit.Thiscorrespondsto400–650molesofRNAforeachmoleoftemplate.Smallertemplatestypicallyyieldalowermassandahighermolaryieldofproduct.Noveltranscriptionreactionconditionsandapatented,high-yieldtechnologyallowthesynthesisof10–50timestheamountofRNAproducedbyconventionaltranscriptionreactions.ApplicationsTheMEGAscript™T7KitisintendedforthesynthesisoflargeamountsofunlabeledorlowspecificactivityRNAforavarietyofuses,includinginvitrotranslation,antisense/microinjectionstudies,andisolationofRNAbindingproteins.Inlarge-scaletranscriptionreactions,theconcentrationofall4nucleotidesishigh,wellabovetheKmfortheenzyme.TheMEGAscript™T7Kittypicallyyieldsover10timesmoreRNAthanconventionalinvitrotranscriptionreactions.Thekitisnotrecommendedforsynthesisofhighspecificactivityprobes.AccessoryproductsMessageAmp™aRNAamplificationkitsarepoweredbyMEGAscript™technologyandhavebeendevelopedespeciallyfortheamplificationofcRNApriortoarrayanalysis.Topurifyinvitrotranscriptionproductsfromfreenucleotides,buffercomponents,andenzymes,werecommendusingtheMEGAclear™Kit(Cat.No.AM1908)andtheMEGAclear™-96Kit(Cat.No.AM1909).ForResearchUseOnly.Notforuseindiagnosticprocedures.SpecificationsFinalProductTypesyntheticmRNA,mRNAFormatKitSampleTypemRNA,syntheticmRNAForUseWith(Application)InVitroTranscription,syntheticmRNA,mRNA,transfection,LNPs,GENEart,linearizedDNAplasmid,delivery,PCRproductsNo.ofReactions40ReactionsProductLineAmbion™,MEGAscript™ProductTypeT7InVitroTranscriptionkitQuantity40ReactionsPromoterT7ShippingConditionDryIceYield100µgStartingMaterialPCRProducts,LinearizedDNAplasmidContents&StorageT7EnzymeMix,10XReactionBuffer,ATPSolution,CTPSolution,GTPSolution,UTPSolution,pTRI-Xef,TURBODNase,AmmoniumAcetateStopSolution,LithiumChloridePrecipitationSolution,andGelLoadingBufferIIareallstoredat–20°C.Nuclease-freeWatermaybestoredatanytemperature. 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