Plasmid DNA Isolation | Thermo Fisher Scientific - TW

Plasmid isolation and purification are essential steps to many procedures in molecular biology laboratories including DNA sequencing, gene therapy, ... PopularApplications&TechniquesShopAllProductsServicesSupport Signin QuickOrder     Search ThermoFisherScientific SearchAll Search Home›LifeSciences›DNA&RNAExtraction&Analysis›PlasmidDNAIsolationPlasmidDNAIsolationSeeNavigation‹DNA&RNAExtraction&AnalysisAutomatedExtractionforDNA,RNA,Protein&Cells ›GenomicDNAExtractionandGenomicDNAIsolation ›PlasmidDNAIsolation PlasmidDNAMaxiprepKits PlasmidDNAMidiprepKits PlasmidDNAMiniprepKits BenchPro2100PlasmidPurificationSystem PlasmidDNAPurityGrades PureLinkHiPureExpiPlasmidPurificationKits Low-EndotoxinPlasmidDNAPurificationandIsolationKits Endotoxin-FreePlasmidDNAIsolationKits RNAExtractionKitsandRNAIsolationReagents ›ViralNucleicAcidIsolationKits ›RNA/DNAQuantification ›NucleicAcidGelElectrophoresis ›NucleicAcidLabeling ›DNA&RNAPurification&AnalysisEquipment&Plasticware ›NucleicAcidIsolationProtocolVideos ›NucleicAcidIsolationBrochures,ManualsandGuides ›NucleicAcidIsolationApplicationNotes ›SamplePrepandMolecularBiologyIVD-RTransitionUpdates ›DNA&RNAExtractionArticles,WebinarsandVideos ›End-to-EndCancerWorkflows ›Viral/PathogenSamplePreparation ›IsolatePlasmidDNAforEveryApplicationOurInvitrogenplasmidisolationkitsofferoptionstoeliminatethechallengesraisedbymultisampleprocessing.WedesignourplasmidDNAextractionproductstoisolateplasmidDNAatthepurityandscaleyouneed.Choosefromourwiderangeofhigh-performing,cost-effectiveplasmidpurificationtechnologiesdesignedtohelpyouovercomecommonplasmidprepissues,suchaslowrecoveryandthepresenceofimpurities. LearnabouthowtopurifyplasmidDNAwithInvitrogenPureLinkandThermoScientificGeneJETextractionkitswithourplasmidisolationprotocolvideos.RobustpurificationofplasmidDNA,intheamountandpurityrequiredforthedownstreamapplicationofinterest,isthekeystepofproteinexpressionworkflow.LearnmoreabouthowyoucanobtaintransfectiongradeplasmidandusethemwithGibcoExpiCHOandExpi293transientmammalianexpressionsystems.DNALearningCenter Downloadapplicationnote Downloadselectionguide WhichplasmidDNAisolationandpurificationkitisrightforyou?  RapidisolationofmolecularbiologyqualityGoldstandardforultrapure,highqualityEnhancedpurityforsensitiveapplicationsPuritygradeMolecularTransfectionAdvancedtransfectionEndotoxinStandard(>10EU/µg)Lowendotoxin(0.1–1EU/µg)Endotoxin-free(<0.1EU/µg)PlasmidDNApurificationkitGeneJETPureLinkHiPurePureLinkFastPureLinkExpiEndotoxin-FreeTotalprotocoltime15–60min30–120min30min90–120minPrepsizeMini–MaxiMini–GigaMidi,MaxiMaxi,Mega,GigaYieldupto20µg–1mg20µg–15mg0.4mg(midiprep)or1.5mg(maxiprep)1.5–15mgTechnologySilicamembraneAnionexchangeAdvancedsilicaAnionexchangemembraneApplicationsNotsensitivePCRCloning(digestion,ligation)SequencingSensitive  Transfectionofstandard,robustcelllinesAllmolecularbiologyapplicationsVerysensitivePrimaryandstemcelltransfectionGenetherapyandvaccine(invivo)researchAllstandardtransfectionapplicationsAllmolecularbiologyapplications OrdernowOrdernowOrdernowOrdernow PlasmidDNAPreparationSizes  MiniprepMidiprepMaxiprepMegaprepGigaprepOvernightbacterialculturevolume1–5mL25–100mL100–500mL500mL–2.5L2.5–5LApproximateyieldUpto40µgUpto400µgupto1.5mgUpto5mgUpto15mg ExtractPlasmidDNAwithPureLinkandGeneJet Howtopurifyendotoxin-freeplasmidDNA WatchthisvideotolearnhowtopurifyplasmidDNAusingthePureLinkExpiEndotoxin-FreeMaxiPlasmidPurificationKit.Thisvideoshowsyouhowtoisolateandpurifyhigh-quality,endotoxin-freeplasmidDNAforsensitiveapplicationslikeprimarycelltransfection. Howtopurifymolecular-gradeplasmidDNA Thisvideoshowsyouhowtopurifymolecular-gradeplasmidDNAin15minutesusingourGeneJETPlasmidMiniprepKit.Molecular-gradeplasmidDNAissuitableforcloning,nucleicacidlabeling,PCR,DNAsequencing,andtransformation. HowtopurifyplasmidDNAusingourmegaprepkit Isolatetransfection-gradeplasmidDNAinaslittleas90minuteswiththeInvitrogenPureLinkHiPureExpiPlasmidMegaprepKit.Thisinnovativeproprietaryanion-exchangeresininthesekitscombineslargecapacitywithfastflowrate,highresolution,andefficiency. HowtopurifytransfectiongradeplasmidDNA LearnhowtopurifytransfectiongradeplasmidDNAwiththeInvitrogenPureLinkHiPurePlasmidFilterMaxiprepKit.TransfectiongradeplasmidDNAissuitableforallmolecular-gradeapplications,aswellasinvitrotranscriptionandtransfection. Shopforeverydaylabessentialstopoweryourtransfectionsteps We'vemadeiteasierforyoutoquicklyfindandbuyproductsforeverystepinyourtransfectionworkflow.Shopnow Resources NucleicAcidPurificationandAnalysisSupport CenterFindtips,troubleshootinghelp,andresourcesforyournucleicacidpurification&analysisapplications.DNA&RNALearningCenterDNAPurificationandAnalysisGuideRNAPurificationandAnalysisGuideKingFisherInstrumentsGuideDynabeadsMagneticBeadsGuideSeeallnucleicacidisolationbrochures,manualsandguidesSARS-CoV-2testingWhitePaper:End-to-endworkflowsolutionsforlow-tohigh-throughputSARS-CoV-2testingGuide:SARS-CoV-2supportandsolutionsKingFisherinstrumentsandMagMAXisolationkitsApplicationNote:MagMAXViral/PathogenkitprotocolchangestoenableincreasedSARS-CoV-2testingthroughputProtocolVideos—Videostohelpyouisolatenucleicacidsusingavarietyoftechniques.DNA&RNASelectionGuide—Findtherightpurificationproducts.Videos—ViewourlibraryofDNA&RNApurificationandanalysisvideos.ApplicationNotes—ExploreourapplicationnotesfromscientistssharingdataforisolationproductsForResearchUseOnly.Notforuseindiagnosticprocedures. 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