Plasmid DNA Transfection | Thermo Fisher Scientific - TW

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Plasmids are small circular DNA molecules that naturally occur in bacteria, and are actually used by the bacteria to transfer genetic information. The mechanism ... PopularApplications&TechniquesShopAllProductsServicesSupport Signin QuickOrder     Search ThermoFisherScientific SearchAll Search Home›LifeSciences›CellCulture&Transfection›TransfectionEssentials›PlasmidDNATransfectionPlasmidDNATransfectionSeeNavigation‹TransfectionEssentialsTransfectionReagents—DNA,RNA&Protein ›PlasmidDNATransfection ›TransfectionSolutionsforCancerCells ›siRNATransfection ›TransfectionMethods ›SelectionAntibiotics ›TransfectionProtocols&Support ›NeonElectroporationTransfection ›Toobtainefficientgenetransferbytransfection,plasmidDNAcanbecomplexedwithlipidreagentstomediateefficientdeliveryintothecell'snucleus.Thisprocessisessentialforsubsequentproteinexpressionofthegeneofinterest.PlasmidsaresmallcircularDNAmoleculesthatnaturallyoccurinbacteria,andareactuallyusedbythebacteriatotransfergeneticinformation.ThemechanismofaddingaDNAplasmidintoamammaliancellisknownasplasmidtransfection.OurInvitrogenLipofectamineReagent–specificprotocolshavebeenoptimizedforefficiency,viability,andreproducibilityacrossabroadrangeofcelltypes(see"Downloadprotocol"linksinthetablebelow).Thisisoftenthebestplacetostart,especiallyinanewcellline.Ifyoufindthisdoesn’tworkforyouspecificcelltype,thenyoucanyoulooktoourcellspecificprotocolsforfurtheroptimization. InvitrogenTransfectionSelect™productselectiontool Answerafewsimplequestionsaboutyourtransfection,andthetoolquicklyandaccuratelygeneratesrecommendedsolutions,protocols,citationstomeetyourresearchneeds.TrythetoolWhichInvitrogenplasmidDNAtransfectionreagentproductisrightforyou? High-efficiency,versatilereagentforawiderangeofcommoncelltypesLipofectamine2000Learnmore›Mostefficientversatilereagentforthewidestrangeofcelltypesincludingdifficult-to-transfectcellsLipofectamine3000Learnmore›High-efficiencyelectroporationforallcelllines;bestforprimary&stemcellsNeonTransfectionSystemLearnmore›Top-sellingformatCat.No.11668-019(1.5mL)Cat.No.L3000-015(1.5mL)Cat.No.MPK5000SSampletypeDNARNACo-transfectionDNARNACo-transfectionDNARNACo-transfectionProteinTransfectionefficiencyHighSuperiorMaximalCellviabilityHighSuperiorGoodProtocolDownloadprotocolDownloadprotocolDownloadprotocol OrdernowOrdernowOrdernow OtherplasmidDNAtransfectionreagents 293fectinTransfectionReagentCellfectinII(transfection)ReagentFreeStyleMAX(transfection)ReagentOptifectTransfectionReagentCalciumphosphateResourcesTransfectiontechniquesandbasicsCationiclipidtransfectionoverviewHowcationiclipidmediatedtransfectionworksSupportTransfectionsupportcenterFindtechnicalsupportrecommendationsforyourlipid-basedtransfectionworkflowsandforelectroporationmethods,includingtipsforexperimentalsetupandin-depthtroubleshootinghelp.Cell-specifictransfectionprotocolsForResearchUseOnly.Notforuseindiagnosticprocedures. 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