小腦經視丘至前扣帶迴皮質的神經連結

本研究在大鼠腦中注射神經追蹤劑，來觀察小腦及和情緒功能高度相關的大腦邊緣系統之間的 ... CL),中央內側核(central medial thalamic nucleus, CM)等位於視丘的核區， ... 資料載入處理中... 跳到主要內容 臺灣博碩士論文加值系統 ::: 網站導覽| 首頁| 關於本站| 聯絡我們| 國圖首頁| 常見問題| 操作說明 English |FB專頁 |Mobile 免費會員 登入| 註冊 功能切換導覽列 (165.22.106.144)您好！臺灣時間：2022/05/2807:16 字體大小：       ::: 詳目顯示 recordfocus 第1筆/ 共1筆  /1頁 論文基本資料 摘要 外文摘要 目次 參考文獻 紙本論文 QRCode 本論文永久網址: 複製永久網址Twitter研究生:吳奕君研究生(外文):I-ChunWu論文名稱:小腦經視丘至前扣帶迴皮質的神經連結論文名稱(外文):Ananatomicalstudyofcerebellumprojectiontoanteriorcingulatecortexthroughthalamus指導教授:嚴震東指導教授(外文):Chen-TungYen口試委員:陳瑞芬、蔡孟利、高淑芬、賴文崧口試委員(外文):Ruei-FengChen、Meng-LiTsai、Shur-FenGau、Wen-SungLai口試日期:2015-01-26學位類別:碩士校院名稱:國立臺灣大學系所名稱:生命科學系學門:生命科學學門學類:生物學類論文種類:學術論文論文出版年:2015畢業學年度:103語文別:英文論文頁數:74中文關鍵詞:小腦深部核、邊緣系統、神經追蹤劑、視丘內背側核、板內側核外文關鍵詞:deepcerebellarnuclei、limbiccortex、neuronaltracer、mediodorsalthalamicnucleus、intralaminarnuclei相關次數: 被引用:0點閱:347評分:下載:0書目收藏:0 小腦除了在運動功能上扮演重要角色，近期行為及電生理研究也發現小腦參與了認知和情緒等功能，但在解剖上的證據較不明確。

本研究在大鼠腦中注射神經追蹤劑，來觀察小腦及和情緒功能高度相關的大腦邊緣系統之間的連結。

本實驗分為四個部分：(1)在大鼠小腦深部核(deepcerebellarnuclei,DCN)注射順行性神經追蹤劑;(2)在大鼠視丘中央部位注射反行性神經追蹤劑;(3)在大鼠前扣帶迴皮質區(anteriorcingulatecortex,ACC)注射反行性神經追蹤劑;(4)同時在大鼠小腦注射順行性神經追蹤劑，並在大腦邊前扣帶迴皮質區注射反行性神經追蹤劑。

使存活一段時間後，將動物犧牲灌流並將腦固定後取出，以冷凍切片並進行觀察。

在此研究中發現:(1)小腦除了投射至腹前側核(ventralanteriorthalamicnucleus,VA),腹外側核(ventrolateralthalamicnucleus,VL),腹內側核(ventromedial,VM)等運動相關核區，也會投射到內背側核外側部分(lateralpartofmediodorsalthalamicnucleus,MDL),中央旁核(paracentralthalamicnucleus,PC),中央外側核(centrolateralthalamicnucleus,CL),中央內側核(centralmedialthalamicnucleus,CM)等位於視丘的核區，其中以齒狀核(dentatenuclei,DN)的投射最多。

(2)靠近尾端的內背側核,板內側核(intralaminarnuclei,ILN)等視丘核區傾向投射到靠近頭側的前扣帶迴皮質區，而較頭側的視丘核區則會投射到靠近尾端的前扣帶皮質區。

(3)在小腦及前扣帶迴皮質區分別注射順行及反行性的神經追蹤劑實驗中，發現在小腦齒狀核及中間核(interposednuclei,IN)注射追蹤劑的大鼠中，可以在視丘內背側核外側部分,中央旁核,中央外側核等區域發現被標定的神經細胞被從小腦投射而來的軸突末端包圍。

說明小腦中神經元可能通過丘腦中的這些核區作為中繼站進而和前扣帶迴皮質區進行連結與功能協調。

本結果在解剖上提供小腦在認知、情緒等非運動功能上也扮演了協調的可能。

Cerebellumhaslongbeenthoughtasamainareaformotorexecutivecontrol.Recentstudiesofelectrophysiologyandbehaviorrevealthatcerebellumalsoparticipatesincognitiveandemotionalfunctions.However,thereisstillnosufficientanatomicalevidence.Thestudyincludesfourparts:we(1)injectedanterogradeneuronaltracerintheratdeepcerebellarnuclei(DCN);(2)injectedretrogradetracerinthemidthalamus;(3)injectedretrogradetracerinanteriorcingulatecortex(ACC);(4)simultaneouslyinjectedanterogradetracerintheDCNandretrogradetracerinACC.Afterseveraldaysofsurvival,theanimalwassacrificedforperfusionandthebrainwasfixedandremovedforfurtherfrozensection.Theresultsshow:(1)Exceptforventralanteriorthalamicnucleus(VA),ventrolateralthalamicnucleus(VL)andventromedialthalamicnucleus(VM),cerebellaralsoprojectstonon-motornuclei,suchaslateralpartofmediodorsalthalamicnucleus(MDL),paracentralthalamicnucleus(PC),centrolateralthalamicnucleus(CL)andcentralmedialthalamicnucleus(CM),anddentatenuclei(DN)isthemainoutput.(2)TheretrogradetracingofACCinjectionoffluoro-goldshowsatendencythatrostralmedialthalamuspreferentiallyprojectstoA2andcaudalmedialthalamustoA4levelofACC.(3)InMDL,PC,andCL,manyretrogradetracerlabeledneuronscanbeseenandanterogradetracerlabeledaxonfiberandboutonsaroundthoseneurons.Ourresultsindicatethatthecerebellummayhaveconnectionswithanteriorcingulatecortexthroughmediodorsalthalamicnucleus,paracentralnucleus,orcentrallateralnucleusandprovideanatomicaldatashowingtheroleofemotionalcoordinationbythecerebellum. TableofContents誌謝………………………………………………………………………ii摘要…………………………………………………………………iiiAbstract………………………………………………………………ivAbbreviations……………………………………………………………11.Introduction……………………………………………………….21.1Cerebellumandnon-motorfunctions………………………………………21.2Cerebellumoutputandconnectionswiththecerebrum……………………31.3Anteriorcingulatecortexanditsconnections……………………………..41.4Connectionsofcerebellumandanteriorcingulatecortex……………………….61.5Aimsandexperimentaldesign………………………………………………….72.MaterialsandMethods……………………………………………82.1Animals…………………………………………………………………….82.2Surgery…………………………………………………………………….82.3.Histology………………………………………………………………...102.4DataAnalysis……………………………………………………………..113.Results…………………………………………………………….133.1Anterogradetracingfromdeepcerebellarnucleitothalamus…………….133.2RetrogradetracingfromMDandIntralaminarnucleitoDCN……………143.3RetrogradetracingfromACCtothalamus…………………………………153.4DoubletracingofACCandDCN…………………………………………174.Discussions………………………………………………………..19References……………………………………………………………25Tables…………………………………………………………………32Figures………………………………………………………………..35 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