pGEM®-T Easy Vector Systems - Promega Corporation

The pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a ... Promega'sCookiePolicy Weusecookiesandsimilartechnologiestomakeourwebsitework,runanalytics,improveourwebsite,andshowyoupersonalizedcontentandadvertising.Someofthesecookiesareessentialforourwebsitetowork.Forothers,wewon’tsetthemunlessyouacceptthem.Tofindoutmoreaboutcookiesandhowtomanagecookies,readourCookiePolicy. AcceptAll YourAccount Username Accountnotfound. Emailaddressisunverified. Accountislocked. Accountislocked. Thisfieldisrequired. Password Incorrectpassword. Passwordresetisrequired. Thisfieldisrequired. Accountisinvalid. Unabletologin. LogIn ForgotPassword? CreateanAccount Toprotectyourprivacy,youraccountwillbelockedafter6failedattempts.Afterthat,youwillneedtocontactCustomerServicetounlockyouraccount. Youhave4remainingattempts. Youhave3remainingattempts. Youhave2remainingattempts. Youhave1remainingattempt. 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FindSalesContact About AboutPromega CompanyInformation Newsroom CorporateCampusMap JoinOurTeam CareerOpportunities Sustainability CorporateResponsibility CommunityTouch PlanetAware PeopleCare CorporateMind ProductReach ContactUs CustomerSupport YourCart CurrentItems0 ViewCart Checkout Addmultipleproducts Home Products PCR PCRCloning pGEM®-TEasyVectorSystems Share ConvenienceandFlexibilityforCloningPCRProducts Threeoptionsforremovingtheinsertwithasingledigest(BstZI,EcoRIorNotI) Ligationcanbecompletedin1houratroomtemperature Availablewithorwithoutcompetentcells Size 20reactions Addcompetentcells AddJM109CompetentCells Catalognumberselected: A1360 AddtoCart Thisproductisdiscontinued AddtoHelixThisproductisavailablethroughthePromegaHelixonsitestockingprogram.Weoffernumerousconvenientsolutionstomeetyourlab'sneeds.LearnmoreabouttheHelixCollection» ThisproductisavailableunderourEarlyAccessprogram-LearnMore ThisproductisavailableunderourCatalog(FT)program-LearnMore pGEM®-TEasyVectorSystems 20reactions Yourprice: ChangeConfiguration Overview Protocols Specifications Resources RelatedProducts Workflows PCRCloningwithBlue/WhiteSelectionandEasyInsertExcision ThepGEM®-TEasyVectorSystemsareconvenientsystemstoclonePCRproductsgeneratedbycertainthermostablepolymerases.Thesepolymerasesoftenaddasingledeoxyadenosine,inatemplate-independentfashion,tothe3´-endsoftheamplifiedfragments.ThepGEM®-TEasypre-linearizedVectorcontains3´-ToverhangsattheinsertionsitetoprovideacompatibleoverhangforPCRproducts.Theprovided2XRapidLigationBufferallowsreactionstobecompletedin1houratroomtemperature.Theincubationperiodmaybeextendedtoincreasethenumberofcoloniesaftertransformation.  T7andSP6RNApolymerasepromotersflankamultiplecloningregionwithintheα-peptidecodingregionforβ-galactosidase.Insertionalinactivationoftheα-peptideallowsrecombinantclonestobedirectlyidentifiedbyBlue/WhiteScreeningonindicatorplates.Thevectorallowspreparationofsingle-strandedDNAduetoitsf1OriginofReplication. ThepGEM®-TEasyVectorSystemsofferalloftheadvantagesofthepGEM®-TVectorSystemswiththeaddedconvenienceofrecognitionsitesforBstZI,EcoRIandNotIflankingtheinsertionsite.Thus,severaloptionsexisttoremovethedesiredinsertDNAwithasinglerestrictiondigestion. ThepGEM®-TEasyVectorSystemIIcontainsJM109CompetentCellsinadditiontoallofthepGEM®-TEasyVectorSystemIcomponents. ThereisnoGenBank®AccessionNumberforthepGEM®-TEasyVector.The parentvectorislinearizedatthepositionindicatedby*inthis pGEM®-TEasyVectorSequence anda"T"is addedateachend. Protocols CompleteProtocol pGEM®-TandpGEM®-TEasyVectorSystemsTechnicalManualPDF(724KB)–English QuickProtocols pGEMTandpGEMTEasyVectorSystemsFB033PDF(202KB)–English VideoProtocols Specifications Youareviewing: A1360 ChangeConfiguration What'sinthebox? Item Part# Size Concentration AvailableSeparately pGEM®-TEasyVector A137A 1×1.2μg 50ng/μl ControlInsertDNA A363A 1×12μl 2XRapidLigationBuffer C671A 1×200μl ViewProduct T4DNALigase M180A 1×100u SDS SearchforSDS CertificateofAnalysis Searchbylotnumber UseRestrictions ForResearchUseOnly.NotforUseinDiagnosticProcedures. StorageConditions SeeProtocolfordetailedstoragerecommendations. Specifications Youareviewing: A1380 ChangeConfiguration What'sinthebox? Item Part# Size Concentration AvailableSeparately pGEM®-TEasyVector A137A 1×1.2μg 50ng/μl ControlInsertDNA A363A 1×12μl T4DNALigase M180A 1×100u 2XRapidLigationBuffer C671A 1×200μl ViewProduct JM109CompetentCells,HighEfficiency L200A 6×200μl SDS SearchforSDS CertificateofAnalysis Searchbylotnumber UseRestrictions ForResearchUseOnly.NotforUseinDiagnosticProcedures. StorageConditions SeeProtocolfordetailedstoragerecommendations. Resources Articles MeasuringGeneExpressionfromMammalianBrainTissue RapidLigationforthepGEM®-TandpGEM®-TEasyVectorSystems ComparingCloningEfficiencyofthepGEM®-TandpGEM®-TEasyVectorstotheTOPOTACloning®Vectors Citations Rolesof14-3-3βandγinregulationoftheglucocorticoidreceptortranscriptionalactivationandhepaticgluconeogenesis. -- 2018 Biochem.Biophys.Res.Commun. Seeallcitations SimilarProducts pGEM®-TVectorSystems T-vectorforsimplifiedcloningofPCRproducts. A3600,A3610 pTargeT™MammalianExpressionVectorSystem PCRcloningsystemforexpressioninmammaliancells. A1410 FrequentlyUsedWith GoTaq®G2MasterMixes Second-generation,high-performanceGoTaq®G2DNAPolymeraseinaready-to-usemastermix. M7822,M7823,M7832,M7833 GoTaq®G2DNAPolymerase LatestgenerationGoTaq®polymerase—high-performanceforyoureverydayPCRneeds. M7841,M7845,M7848 GoTaq®G2FlexiDNAPolymerase Second-generation,high-performanceGoTaq®G2DNAPolymerasewithMg-freebuffers. M7801,M7805,M7806,M7808 GoTaq®G2HotStartPolymerase High-performancehot-startGoTaq®G2enzymewithconvenientroom-temperaturesetup. M7401,M7405,M7406,M7408 GoTaq®G2HotStartMasterMixes Ready-to-useoptimizedmastermixforroom-temperaturePCRassembly.ContainsGoTaq®G2enzyme. M7422,M7423,M7432,M7433 GoTaq®LongPCRMasterMix Hot-startmastermixidealforlong-rangePCR. M4021 Seeall Let'sfindtheproductthatmeetsyourneeds. 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