Vector Database - pGEM-T Easy Vector - Addgene

The MCS of the pGEM-T Easy Vector contains sequences on either side of the insert that are recognized by the restriction enzymes Not I and EcoR I. This allows ... Skiptomaincontent VectorDatabaseHomepage pGEM-TEasyVector VectorDatabase WelcometoVectorDatabase! Vectordatabaseisadigitalcollectionofvectorbackbonesassembledfrompublicationsandcommerciallyavailablesources.ThisisafreeresourceforthescientificcommunitythatiscompiledbyAddgene. Thispageisinformationalonly-thisvectorisNOTavailablefromAddgene-pleasecontactthemanufacturerforfurtherdetails. Searchvectordatabase ThisvectorisNOTavailablefromAddgene. Plasmid:pGEM-TEasyVector Source/Vendor: Promega AltName: pGEM®-TEasy,pGEM-TEasy Analyze: Sequence PlasmidType: BacterialExpression ExpressionLevel: High CloningMethod: Unknown Size: 3015 5'Sequencing1Primer: T7,SP6,M13FwdorM13Rev BacterialResistance: Ampicillin Notes: TheonlydifferencebetweenpGEM-TandpGEM-TEasyisinthemultiplecloningsite(MCS).TheMCSofthepGEM-TEasyVectorcontainssequencesoneithersideoftheinsertthatarerecognizedbytherestrictionenzymesNotIandEcoRI.ThisallowstheinsertDNAtoberemovedwithasinglerestrictiondigestusingeitheroftheseenzymes. ParentalvectorforTAcloningofPCRproducts. CatalogNumber: A1360 Stable: Transient Constitutive: Constitutive Viral/Non-Viral: Nonviral GeneratedPlasmidMap Loading... AnalyzeSequence