Difference Between Inducible and Constitutive Promoter

Inducible and constitutive promoters are two such types. Inducible promoters are regulated promoters that become active in the cell only in ... Home/Science&Nature/Science/Biology/MolecularBiology/DifferenceBetweenInducibleandConstitutivePromoter Thekeydifferencebetweeninducibleandconstitutivepromoteristhattheinduciblepromoterisaregulatedpromoterthatisactiveonlyinresponsetospecificstimuliwhiletheconstitutivepromoterisanunregulatedpromoterwhichisactiveinallcircumstances. Promoterisanessentialpartofagene.ItisaDNAsequencelocatednearandupstreamtothetranscriptioninitiationsiteofthegene.Promotersmayhave100to1000basepairs.TheyallowthebindingofRNApolymerase(enzyme)andothernecessaryproteins(transcriptionfactorswhichrecruitRNApolymerase)tostarttranscription.Therearedifferenttypesofpromoters.Inducibleandconstitutivepromotersaretwosuchtypes.Induciblepromotersareregulatedpromotersthatbecomeactiveinthecellonlyinresponsetoaspecificstimulus.Ontheotherhand,constitutivepromotersareunregulatedpromotersthatareactiveinallcircumstancesinthecell. CONTENTS 1. OverviewandKeyDifference 2. Whatisan InduciblePromoter 3. WhatisaConstitutivePromoter 4. SimilaritiesBetweenInducibleandConstitutivePromoter 5. SidebySideComparison–PrimervsPromoterinTabularForm 6. Summary WhatisanInduciblePromoter? Induciblepromoterisaregulatedpromoterwhichbecomesonoractiveinthecellinresponsetospecificstimuli.Therefore,thesepromotersareactiveonlyundercertaincircumstances.Unlessitreceivesastimulus,induciblepromoterstaysatinactivestate.Intheinactivestateoroffstate,transcriptionalfactorsorRNApolymerasecannotbindwiththepromoter.Onceaninducerbindstotheactivatorprotein,activatorproteinbindswiththepromoterandmakesitactivetoinitiatetranscription. Induciblepromotersactivateinresponsetodifferentstimulisuchaslight,temperatureandchemicalagents,etc.Basedonthetypeofstimulusthatactivatesthepromoter,therearedifferenttypesofinduciblepromotersaschemicallyinduciblepromoters,inducibletemperaturepromotersandlight-induciblepromoters,etc.Chemicallyinduciblepromotersarethemostcommon.Someinduciblepromotersareactivatedbyalcohols,tetracycline,antibiotics,copper,steroid,etc.Temperatureinducible,light-inducible,oxygenlevel,cold-inducible,heat-inducibleandmechanicalinjuryinduciblepromotersarealsocalledphysicallyinduciblepromoters. Figure01:Promoter WhatisaConstitutivePromoter? Constitutivepromoterisanunregulatedpromoterwhichisactiveinvivoatallthecircumstances.Therefore,thesepromoterscarryoutthetranscriptionofassociatedgenescontinuouslyinthecell.Theactivitiesofthesepromotersarenotaffectedbytranscriptionalfactors.Regardlessofthesurroundingenvironmentandthedevelopmentstageoftheorganism,constitutivepromotersallowthetranscriptionofgenesinalltissues.Theyarelargelyindependentofenvironmentalanddevelopmentalfactors.Hence,thesepromotersareactiveacrossspeciesandevenacrosskingdoms. Inplants,constitutivepromotersareusedfortheexpressionoftransgenes.Generally,acellrequirestRNA,rRNA,RNApolymerase,ribosomalproteinscontinuously.Thegenesthatproducetheseproductshaveconstitutivepromoters.Someexamplesofconstitutivepromotersincludeplantubiquitinpromoter,riceactin1promoter,maizealcoholdehydrogenasepromoter,etc.OpinepromoterandCaMV35Spromoteraretwomoreexamplesofconstitutivepromoters. WhataretheSimilaritiesBetweenInducibleandConstitutivePromoter? Inducibleandconstitutivepromotersaretwoofthefourtypesofpromoters. TheyareDNAsequenceslocatednearthetranscriptioninitiationsiteofgenes. TheyfacilitatethebindingofRNApolymeraseandtranscriptionfactors. WhatistheDifferenceBetweenInducibleandConstitutivePromoter? Induciblepromoterisaregulatedpromoterthatallowstranscriptionofitsassociatedgenesonlyincertaincircumstancesinthecell.Constitutivepromoterisanunregulatedpromoterwhichallowscontinualtranscriptionofitsassociatedgenes.So,thisisthekeydifferencebetweeninducibleandconstitutivepromoter.Moreover,anotherdifferencebetweeninducibleandconstitutivepromoteristhattheinduciblepromotersareaffectedbyabioticandbioticfactors,whiletheconstitutivepromotersarelargelyindependentofenvironmentalanddevelopmentalfactors. Belowinfo-graphicsummarizesthedifferencesbetweeninducibleandconstitutivepromoterintabularform. Summary– InduciblevsConstitutivePromoter Inducibleandconstitutivepromotersaretwotypesofpromoters.Induciblepromotersallowthetranscriptionoftheirgenesonlyatcertaincircumstancesinresponsetospecificstimuli.Theyareregulatedpromoters.Ontheotherhand,constitutivepromotersallowcontinuoustranscriptionoftheirassociatedgenes.Theyareunregulatedpromoters.Thus,thisisthekeydifferencebetweeninducibleandconstitutivepromoter.Constitutivepromotersareindependentofenvironmentalanddevelopmentalfactorswhileinduciblepromotersareaffectedbythebioticandabioticfactors. Reference: 1.Gearing,Mary.“Plasmids101:InduciblePromoters.”AddgeneBlogShareScience,Availablehere. 2.“Promoter(Genetics).”Wikipedia,WikimediaFoundation,27Nov.2020,Availablehere. ImageCourtesy: 1.“Figure160201”  ByCNXOpenStax–(CCBY4.0)viaCommonsWikimedia Relatedposts: DifferenceBetweenTranslocationandCrossingOver DifferenceBetween16srRNAand16srDNA DifferenceBetweenTelomeresandTelomerase DifferenceBetweenHierarchicalandWholeGenomeShotgunSequencing DifferenceBetweenRandomPrimersandOligodT AbouttheAuthor:Dr.SamanthiDr.SamanthiUdayanganiholdsaB.Sc.DegreeinPlantScience,M.Sc.inMolecularandAppliedMicrobiology,andPhDinAppliedMicrobiology.HerresearchinterestsincludeBio-fertilizers,Plant-MicrobeInteractions,MolecularMicrobiology,SoilFungi,andFungalEcology. 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