Genomic DNA from Desulfovibrio desulfuricans subsp ... - ATCC

Genomic DNA isolated from Desulfovibrio desulfuricans subsp. desulfuricans Strain MB. This bacterial strain is also available as ATCC® Catalog No. 27774™. Home MicrobeProducts BacteriologyandArchaea NucleicAcids 27774D-5 Share GenomicDNAfromDesulfovibriodesulfuricanssubsp.desulfuricansstrainMB 27774D-5™ DownloadGenome Learnaboutour EnhancedAuthenticationInitiative GenomicDNAisolatedfromDesulfovibriodesulfuricanssubsp.desulfuricans StrainMB.ThisbacterialstrainisalsoavailableasATCC®CatalogNo.27774™. Productcategory Bacteria Producttype Nucleicacid Derivedfrom Desulfovibriodesulfuricanssubsp.desulfuricansMB(ATCC27774) Organism Desulfovibriodesulfuricans(Beijerinck)KluyverandvanNiel Typestrain No Genomesequencedstrain Yes Applications Bioinformatics Next-generationsequencing Mass 5μg Productformat Freeze-dried Shippinginformation Storedin1XTEbuffer BuyNow Ourproductsareavailablethroughadistributorforyourlocation Determineyourdistributor AddToList Documentation Productsheet Certificateofanalysis Safetydatasheet CertificateofAnalysisDownload TodownloadacertificateofanalysisforGenomicDNAfromDesulfovibriodesulfuricanssubsp.desulfuricansstrainMB(27774D-5),enterthelotnumberexactlyasitappearsonyourproductlabelorpackingslip. Lotnumber GetCertificateofAnalysis CertificateofAnalysisRequest ThecertificateofanalysisforthatlotofGenomicDNAfromDesulfovibriodesulfuricanssubsp.desulfuricansstrainMB(27774D-5)isnotcurrentlyavailableonline.Completethisformtorequestthiscertificateofanalysis. Accountnumber ATCCitemnumber Lotnumber Emailaddress RequestCertificateofAnalysis Nevermind,Idonotneedthiscertificateofanalysis Wehavereceivedyourrequestforthiscertificateofanalysis.Wewillcontactyouassoonaspossible. Ok Youcanfindyouraccountnumberonyoursalesorderconfirmationororderinvoice. CertificateofOriginDownload TodownloadacertificateoforiginforGenomicDNAfromDesulfovibriodesulfuricanssubsp.desulfuricansstrainMB(27774D-5),enterthelotnumberexactlyasitappearsonyourproductlabelorpackingslip. Lotnumber GetCertificateofOrigin CertificateofOriginRequest ThecertificateoforiginforthatlotofGenomicDNAfromDesulfovibriodesulfuricanssubsp.desulfuricansstrainMB(27774D-5)isnotcurrentlyavailableonline.Completethisformtorequestthiscertificateoforigin. Accountnumber ATCCitemnumber Lotnumber Emailaddress RequestCertificateofOrigin Nevermind,Idonotneedthiscertificateoforigin Wehavereceivedyourrequestforthiscertificateoforigin.Wewillcontactyouassoonaspossible. Ok Youcanfindyouraccountnumberonyoursalesorderconfirmationororderinvoice. Thisproductsheetisnotavailableonline.Weonlyprovidethisproductsheettocustomerswhohavepurchasedthisbiosafetylevel3product.Ifyoupurchasedthisproduct,pleasecontactthedistributorfromwhomyoupurchasedthisproductforthisproductsheet. SafetyDataSheetDownload OpentheSafetyDataSheetforthisproducttodownload.  Selectalanguage Language Chinese English French German Italian Japanese Korean Portuguese Spanish error GetSDS Cancel Thissafetydatasheetisnotcurrentlyavailableonline.Pleasecontactusbysubmittingarequestforthissafetydatasheet. ContactUs Haven'tfoundwhatyouneed? Wetailorourmaterialsforyourspecificapplication. Getapplication-specificmaterials BSL1 ATCCdeterminesthebiosafetylevelofamaterialbasedonourriskassessmentasguidedbythecurrenteditionofBiosafetyinMicrobiologicalandBiomedicalLaboratories(BMBL),U.S.DepartmentofHealthandHumanServices.Itisyourresponsibilitytounderstandthehazardsassociatedwiththematerialperyourorganization’spoliciesandproceduresaswellasanyotherapplicableregulationsasenforcedbyyourlocalornationalagencies. RequiredProducts Theseproductsarevitalfortheproperuseofthisitemandhavebeenconfirmedaseffectiveinsupportingfunctionality.Ifyouusealternativeproducts,thequalityandeffectivenessoftheitemmaybeaffected. MolecularGradeWater 60-2450 Detailedproductinformation Expandall Collapseall Characteristics Comments GenomesequencedstrainThispreparationofhighmolecularweightDNAisappropriatefortheuseinthepolymerasechainreaction(PCR)*processandothermolecularbiologyapplications.*ThePCRprocessiscoveredbypatentsownedbyHoffmann-LaRocheInc.UseofthePCRprocessrequiresalicense. Handlinginformation Handlingprocedure CentrifugetubepriortoopeningtopreventlossofpelletedmaterialRehydratecontentsofvialwithmoleculargradeH2O.Placevialat37°Cfor1hourorat+2°Cto8°Covernight.FormorecompleterehydrationandtofullyrecoverDNAincubatethesampleovernightat4°Cwhilerocking;thenincubatefor1hourat65°C. ResuspendingthedriedDNAin≥250µLmaygivebetterresults. Handlingnotes GenomicDNAisolatedfrombacteriaisappropriateforPCR*andothermolecularbiologyapplications.*Thepolymerasechainreaction(PCR)processiscoveredbypatentsownedbyHoffmann-LaRocheInc.UseofthePCRprocessrequiresalicense. Qualitycontrolspecifications Purity(A260/A280) 1.6to2.1 Integrity IntegrityofDNAwasdeterminedbyelectrophoresisona1%agarosegelstainedwithSYBRSafe™,andwasfoundtobeofhighmolecularweight. Functionaltests FunctionalactivitywasconfirmedbyPCRamplificationofthe16SribosomalRNAgene. Identity Identityconfirmedbysequencingof16SribosomalRNAgene(first~500basepairs). Verificationmethod Whole-genomeSequencing History Depositors ATCC Crossreferences GenBankCP001358Desulfovibriodesulfuricanssubsp.desulfuricansstr.ATCC27774,completegenome. Legaldisclaimers Intendeduse Thisproductisintendedforlaboratoryresearchuseonly.Itisnotintendedforanyanimalorhumantherapeuticuse,anyhumanoranimalconsumption,oranydiagnosticuse. Warranty Theproductisprovided'ASIS'andtheviabilityofATCC®productsiswarrantedfor30daysfromthedateofshipment,providedthatthecustomerhasstoredandhandledtheproductaccordingtotheinformationincludedontheproductinformationsheet,website,andCertificateofAnalysis.Forlivingcultures,ATCCliststhemediaformulationandreagentsthathavebeenfoundtobeeffectivefortheproduct.Whileotherunspecifiedmediaandreagentsmayalsoproducesatisfactoryresults,achangeintheATCCand/ordepositor-recommendedprotocolsmayaffecttherecovery,growth,and/orfunctionoftheproduct.Ifanalternativemediumformulationorreagentisused,theATCCwarrantyforviabilityisnolongervalid. Exceptasexpresslysetforthherein,nootherwarrantiesofanykindareprovided,expressorimplied,including,butnotlimitedto,anyimpliedwarrantiesofmerchantability,fitnessforaparticularpurpose,manufactureaccordingtocGMPstandards,typicality,safety,accuracy,and/ornoninfringement. Disclaimers Thisproductisintendedforlaboratoryresearchuseonly.Itisnotintendedforanyanimalorhumantherapeuticuse,anyhumanoranimalconsumption,oranydiagnosticuse.AnyproposedcommercialuseisprohibitedwithoutalicensefromATCC. WhileATCCusesreasonableeffortstoincludeaccurateandup-to-dateinformationonthisproductsheet,ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureandpatentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeenconfirmedtobeaccurateorcompleteandthecustomerbearsthesoleresponsibilityofconfirmingtheaccuracyandcompletenessofanysuchinformation. Thisproductissentontheconditionthatthecustomerisresponsibleforandassumesallriskandresponsibilityinconnectionwiththereceipt,handling,storage,disposal,anduseoftheATCCproductincludingwithoutlimitationtakingallappropriatesafetyandhandlingprecautionstominimizehealthorenvironmentalrisk.Asaconditionofreceivingthematerial,thecustomeragreesthatanyactivityundertakenwiththeATCCproductandanyprogenyormodificationswillbeconductedincompliancewithallapplicablelaws,regulations,andguidelines.Thisproductisprovided'ASIS'withnorepresentationsorwarrantieswhatsoeverexceptasexpresslysetforthhereinandinnoeventshallATCC,itsparents,subsidiaries,directors,officers,agents,employees,assigns,successors,andaffiliatesbeliableforindirect,special,incidental,orconsequentialdamagesofanykindinconnectionwithorarisingoutofthecustomer'suseoftheproduct.Whilereasonableeffortismadetoensureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfromthemisidentificationormisrepresentationofsuchmaterials. Pleaseseethematerialtransferagreement(MTA)forfurtherdetailsregardingtheuseofthisproduct.TheMTAisavailableatwww.atcc.org. References PoweredbyBioz FrequentlyAskedQuestions ExpandALL CollapseALL ViewtheAdditionalProductFAQs ViewallourFAQs RelatedResources Presentations DataProvenance&AuthenticityinMicrobialGenomics AddressingtheNeedforAccuracyandTraceabilityinMicrobialGenomicData:TheATCCGenomePortal CreatingaVaccinefortheTick-bornePowassanVirus AddressingtheNeedforAccuracyandTraceabilityinMicrobialGenomicData:TheATCCGenomePortal TheATCCGenomePortal:OurApproachtoWhole-genomeSequencing RestoringReproducibilityinResearch:FourToolstoAuthenticateYourCells EntericDiseases InfectiousDiseaseResearch ViewAllResources LogIn Emailaddress Password Forgotpassword LogIn Don'thaveaprofile?Createonenow. Youhavepreviouslystartedanaccountapplication.Yourapplicationmaybeinoneofthefollowingstatuses: Incomplete-youdidnotcompleteyourapplicationonline Outforsignature-thesignatureprocessisnotcomplete Underreview-ATCCiscurrentlyreviewingyourapplication YoucancheckthestatusofyourapplicationinyourMyDashboardportal.Youcanselectthe"ContinueAccountApplication"buttonbelowifyouneedtocompleteyourapplication. Youcanalsostartanewapplicationbyselectingthe"Startanewaccountapplication"belowtoestablishanotheraccountwithATCC. Continueaccountapplication Startanewaccountapplication AssociateanAccounttoYourProfile Associatinganaccounttoyourprofileallowsyoutoplaceanorderonatcc.org.Pleaseprovidethefollowinginformationtoaccessthisaccount. Accountnumber Accountbillingpostalcode AssociateAccount You'vesuccessfullyassociatedyouraccountwithyourProfile.YoucanupdateyourdefaultaccountontheMyDashboardAccountsManagementpageatanytime. MyDashboardAccountsManagement Close Youcanfindyouraccountnumberonyoursalesorderconfirmationororderinvoice.