mMESSAGE mMACHINE™ SP6 Transcription Kit

Catalog number: AM1340. Related applications: In Vitro Transcription · Technical SupportCustomer Service. mMESSAGE mMACHINE™ SP6 Transcription Kit. PopularApplications&TechniquesShopAllProductsServicesSupport Signin QuickOrder     Search ThermoFisherScientific SearchAll Search HomeShopAllProductsBiochemicalReagentsTranscriptionandTranslationReagentsmMESSAGEmMACHINE™SP6TranscriptionKitInvitrogen™mMESSAGEmMACHINE™SP6TranscriptionKitCatalognumber:AM1340Relatedapplications:InVitroTranscriptionTechnicalSupportCustomerServiceInvitrogen™mMESSAGEmMACHINE™SP6TranscriptionKitCatalognumber:AM1340Relatedapplications:InVitroTranscriptionTechnicalSupportCustomerServiceCatalogNumberAM1340UnitSize25reactionsPrice(SGD)RequestAQuoteCatalogNumberUnitSizePrice(SGD)AM134025reactionsRequestAQuoteProductOverviewFiguresVideosDocumentsFAQCitations&ReferencesAdditionalInformationRecommendationsRecommendationsmMESSAGEmMACHINE™kitsaredesignedfortheinvitrosynthesisoflargeamountsofcappedRNA.CappedRNAmimicsmosteukaryoticmRNAsfoundinvivo,becauseithasa7-methylguanosinecapstructureatthe5'end.mMESSAGEmMACHINE™kitreactionsincludecapanalog[m7G(5')ppp(5')G]inanultrahigh-yieldtranscriptionreaction.Thecapanalogisincorporatedonlyasthefirstor5'terminalGofthetranscriptbecauseitsstructureprecludesitsincorporationatanyotherpositionintheRNAmolecule.HowmMESSAGEmMACHINE™kitsworkmMESSAGEmMACHINE™kitshaveasimplifiedreactionformatinwhichallfourribonucleotidesandcapanalogaremixedinasinglesolution.Thecapanalog:GTPratioofthissolutionis4:1,whichisoptimalformaximizingbothRNAyieldandtheproportionofcappedtranscripts.mMESSAGEmMACHINE™kitsareidealfortheroutinesynthesisofcappedRNAsforoocytemicroinjection,invitrotranslation,transfection,andotherapplications.ThehighyieldsareachievedbyoptimizingreactionconditionsforRNAsynthesisinthepresenceofhighnucleotideconcentrations.Inaddition,theRNAsynthesizedisprotectedfromdegradationbyanycontaminatingribonucleasesthatmaybepresentwithRNaseinhibitor—acomponentoftheEnzymeMix.UsingmMESSAGEmMACHINE™KitsIna20µLreactionduringa2hourincubation,mMESSAGEmMACHINE™HighYieldCappedRNATranscriptionwillyieldlargemassamountsofcappedRNA.Upto10–50timestheyieldobtainedwithconventionalinvitrotranscriptionreactions.TheratioofcapanalogtoGTPhasbeenoptimizedtoallowthebestcompromisebetweenyield(15–35µg)andproportionoftranscriptsthatarecapped(80%).mMESSAGEmMACHINE™KitsalsocontainaLiClprecipitationsolutionthatisefficientforseparatingproteinsandunincorporatednucleotides(includingfreecapanalog)fromthecappedRNA,allowinganincreasedefficiencyoftranslation.mMESSAGEmMACHINE™kitsareonlyoptimizedforusewiththepolymerasesincludedinthekit.Usingadifferentpolymerasemayresultinlowyields.ThemMESSAGEmMACHINE™kitscontainallthebuffersandreagentsnecessaryfor25transcriptionreactions.Usingthecontroltemplatesuppliedwiththekits(Xenopuselongationfactor1α,pTRIXef),eachmMESSAGEmMACHINE™reactionwillyieldapproximately20–30µgofRNAusingT3orT7RNApolymerase,orabout15–25µgRNAusingSP6RNApolymerase.ForResearchUseOnly.Notforuseindiagnosticprocedures.SpecificationsFinalProductTypesyntheticmRNA,mRNAFormatKitSampleTypemRNA,syntheticmRNAForUseWith(Application)InVitroTranscription,syntheticmRNA,mRNA,transfection,LNPs,GENEart,linearizedDNAplasmid,delivery,PCRproductsNo.ofReactions25ReactionsProductLineAmbion™,mMESSAGEmMACHINE™ProductTypeSP6InVitroTranscriptionkitQuantity25ReactionsPromoterSP6ShippingConditionDryIceYield15-25µgStartingMaterialPCRProducts,LinearizedDNAplasmidContents&StorageSP6EnzymeMix,10XReactionBuffer2XNTP⁄CAPSolution,GTPSolution,pTRI-Xef,TURBODNase,AmmoniumAcetateStopSolution,LithiumChloridePrecipitationSolution,andGelLoadingBufferIIareallstoredat–20°C.Nuclease-freeWatermaybestoredatanytemperature. 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