Ti plasmidEstablish cell lineSingle cell PrinterLonza cell linecell line development中文Cell line developmentClonePixSingle cell cloning protocolHow to seed cultured cells for single clone selection?
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Single clone selection is necessary when performing stable cell line production or drug resistant cells. The goal is to dilute cells down to ... 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Thegoalistodilutecellsdowntoaconcentrationsuchthatwhentheyareplatedinadishthereisasinglecellperwell. Thesinglecellcloneisexpandedbycontinuallyincreasingthewellsizeuntilfreezerstockscanbeestablished.Theoretically,byknowingthestartingcellnumberofthesuspensionascientistcouldsimplymakeasingledilutiondownto1cellperwellandproceed. However,duetopipettingvariancesandslightchangesincellviability,thismethodrarelyproducessuccessfulclones. Thebestrouteforclonalselectionistoplateaslightlyhighercellnumberinawellandthenperform1:2serialdilutionsacrossa96-wellplate. Eventually,oneofthewellswillhaveasinglecell. Keepinmindthatsinglecloneselectionisdifficult,soreplicatesofthedilutionseriesarerecommended. Growthofmultiplesingleclonesallowsresearcherstohavebiologicalreplicatesofresistantcelllinesindownstreamstudies.←Muscle&BoneCellpassage:Howtocorrectlydiluteandsplitculturedcells→Sorry,commentsareclosedforthispost.InVivoTransfectionProducts BrainTransfectionKit Kidney-targetedDeliveryKit Lipid-basedTransfectionKit Liver-targetedDeliveryKit LungsTransfectionKit NanoparticleTransfectionKit Pancreas-targetedDeliveryKit PEG-LiposomeTransfectionKit PolymerTransfectionKitCellLineTransfectionKits CHOTransfectionReagent HepG2TransfectionReagent A549TransfectionReagent DU145TransfectionReagent COS-7TransfectionReagent HeLaTransfectionReagent HEK-293TransfectionReagent LnCAPTransfectionReagent MCF-7TransfectionReagent MDA-MBTransfectionReagent NCI-H460TransfectionReagent SeeallTransfectionProductsPrimaryCellsTransfectionKits AstrocyteTransfectionReagent FibroblastTransfectionReagent HUVECTransfectionReagent Neuro-2aTransfectionReagent SeeallTransfectionProductsElectroporationKits JurkatElectroporationKit K562ElectroporationKit RAW264.7ElectroporationKit SeeallTransfectionProductsTransfectionControls GFP-expressingplasmid CellCycleArrestsiRNA Non-silencingControlsiRNA SeeallTransfectionProductsQUICKLINKS Products|InVivoTransfection|Services|TransfectionResource|HowToOrder|ContactUs
延伸文章資訊
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