跑步運動對不同腦區神經活化之比較

... 區(扣帶皮質、梨狀皮質、中隔內核、海馬回及杏仁核)、基底核(殼/尾狀核、伏隔核心)、間腦(視丘、下視丘)、腦幹(黑質區、腹側被蓋區、上丘、側水管周灰質、中縫背核、 資料載入處理中... 跳到主要內容 臺灣博碩士論文加值系統 ::: 網站導覽| 首頁| 關於本站| 聯絡我們| 國圖首頁| 常見問題| 操作說明 English |FB專頁 |Mobile 免費會員 登入| 註冊 功能切換導覽列 (165.22.106.144)您好！臺灣時間：2022/05/2811:56 字體大小：       ::: 詳目顯示 recordfocus 第1筆/ 共1筆  /1頁 論文基本資料 摘要 外文摘要 目次 參考文獻 電子全文 紙本論文 QRCode 本論文永久網址: 複製永久網址Twitter研究生:劉玉雯研究生(外文):Yu-WenLiu論文名稱:跑步運動對不同腦區神經活化之比較論文名稱(外文):Comparisonoftherunningexercise-inducedneuralactivityindifferentbrainregions指導教授:郭余民指導教授(外文):Yu-MinKuo學位類別:碩士校院名稱:國立成功大學系所名稱:細胞生物與解剖學研究所學門:醫藥衛生學門學類:醫學學類論文種類:學術論文論文出版年:2016畢業學年度:104語文別:英文論文頁數:39中文關鍵詞:跑步機運動、神經活性、c-Fos、BDNF外文關鍵詞:treadmillexercise、neuronalacitvity、c-Fos、BDNF相關次數: 被引用:1點閱:183評分:下載:5書目收藏:0 研究背景：運動已知可提升大腦功能，如:改變神經活性和神經可塑性，這些改變被認為與腦源性神經滋養因子（BDNF）有關。

c-fos是一種即早基因，常被用來標識活性改變的神經細胞。

有研究指出運動會增加大腦中某些腦區的c-Fos蛋白表現量，尤其是海馬回。

然而，運動是否會影響整個腦，或是只有特定腦區，則並不清楚。

研究目的：瞭解那些腦區的神經活性會被跑步運動所影響。

實驗方法：八周大的C57BL/6J小鼠進行一次（急性）、或為期一個月（長期）的中強度跑步機運動，每次一小時。

一部分的急性運動小鼠在跑步後，立即斷頭取腦（以下簡稱E1h組），另一部分則在休息兩小時後，才斷頭取腦（以下簡稱E1hS2h組）。

急性運動控制組的小鼠，則沒有跑步，直接斷頭取腦（以下簡稱Ctrl組）。

長期運動的小鼠，在最後一次跑步後，部分會立即斷頭取腦（以下簡稱1M-E1h組），另一部分則在休息兩小時後，才斷頭取腦（以下簡稱1M-E1hS2h組）。

長期運動控制組的小鼠，則停掉最後一次跑步，與1M-E1h組同時斷頭取腦（以下簡稱1M-Ctrl組）。

腦組織固定後，切成25μm的切片，以組織免疫染色法對c-Fos蛋白進行染色，並在下列腦區進行計數：大腦皮質(運動皮質和體感皮質)、邊緣區(扣帶皮質、梨狀皮質、中隔內核、海馬回及杏仁核)、基底核(殼/尾狀核、伏隔核心)、間腦(視丘、下視丘)、腦幹(黑質區、腹側被蓋區、上丘、側水管周灰質、中縫背核、橋腦核)和小腦(前葉和後葉)。

另一組小鼠腦組織切成一級運動皮質、一級體感皮質、背側海馬回、腹側海馬回、杏仁核、殼/尾狀核、視丘和下視丘，以西方墨點法對BDNF蛋白的表現量進行測量。

結果：相較於Ctrl組，E1h組的c-Fos+細胞密度，在大腦皮質、海馬回及基底核區較高；而到了E1hS2h時，只剩海馬回內的一些區域仍然高於Ctrl組。

在長期運動小鼠，大腦皮質、邊緣區、基底核、間腦及腦幹的側水管周灰質的c-Fos+細胞密度在1M-E1h組高於1M-Ctrl組；到了1M-E1hS2h時，只有海馬回內的一些區域、梨狀皮質、殼/尾狀核、下視丘和側水管周灰質的c-Fos+細胞密度仍然高於1M-Ctrl組。

雖然，急性運動並不影響BDNF蛋白的表現量，但是，BDNF蛋白增加的量和c-Fos+細胞密度增加的量，呈顯著正相關。

結論：無論是急性運動或是長期運動，大腦皮質、海馬回、以及基底核的神經活性都會受到影響而增加；而間腦和腦幹則只會受到長期運動的影響。

急性運動所增加的c-Fos+細胞密度和所增加的BDNF表現量呈正相關。

因此，運動可作為一非藥物、保護大腦的策略，尤其是那些對運動有反應的腦區。

Background:Exercisebenefitsbrainfunctions,suchasincreasesneuronalactivityandsynapticplasticity,whichhasbeenlinkedtobrain-derivedneurotrophicfactor(BDNF).c-fos,animmediateearlygene,isfrequentlyusedasneuronactivationmarker.Exerciseincreasestheexpressionofc-Fosinsomebrainregions,suchashippocampus.However,itisunclearwhethertheeffectofexerciseonbrainisubiquitousorisaregion-specificphenomenon.Objective:Toidentifythebrainregionssensitivetorunning.Methods:C57BL/6Jmice,8-week-old,wereforcedtorunonatreadmillatmoderateintensity,onehoureachtime.Themicewereassignedtothesingle-bout(acute)oronemonth(long-term)exercisegroup.Themicewereeitherkilledimmediately(acute:E1h;long-term:1M-E1h)or2hafter(acute:E1hS2h;long-term:1M-E1hS2h)the1hrunning.Miceoftheacutecontrolgroup(Ctrl)werekilledwithoutrunning.Miceofthelong-termcontrolgroup(1M-Ctrl)omittedthelastrunandkilledatthesametimeasthe1M-E1hgroup.Thebrainswerecutat25μmandimmunostainedforc-Fos.Thenumberofc-Fos+cellswascountedinthefollowingbrainregions:cortex(motorcortex,somatosensorycortex),limbicarea(cingulate,piriformcortex,septalnucleus,hippocampus,amygdala),basalnuclei(caudate/putamen,accumbensnucleuscore),diencephalon(thalamus,hypothalamus),brainstem(substantianigra,ventraltegmentalarea,superiorcolliculus,lateralperiaqueductalgray,dorsalraphenucleus,pontinenucleus)andcerebellum(anteriorandposteriorlobes).Someofthebrainsweredissectintoprimarymotorcortex,primarysomatosensorycortex,dorsalandventralpartsofhippocampus,amygdala,caudate/putamen,thalamusandhypothalamustomeasurethelevelsofBDNF.Results:ComparedtotheCtrlgroup,thedensityofc-Fos+cellswasincreasedinthecortex,hippocampusandbasalnucleiofE1hgroup.IntheE1hS2hgroup,afewregionsofhippocampuswerestillhigherthanthoseoftheCtrlgroup.Inthelong-termexercisemice,thedensityofc-Fos+cellsofcortex,limbicarea,basalnuclei,diencephalonandlateralperiaqueductalgrayofbrainsteminthe1M-E1hgroupwerehigherthanthoseof1M-Ctrlgroup.Thedensityofc-Fos+cellsintheregionsofhippocampus,piriformcortex,caudate/putamen,hypothalamusandlateralperiaqueductalgrayremainedelevatedinthe1M-E1hS2hgroup.AlthoughthelevelsofBDNFwerenotaffectedbyacuteexercise,thechangedlevelsofBDNFwerepositivelycorrelatedwiththechangeddensityofc-Fos+cells.Conclusion:Bothinacuteandlong-termexercisesinfluenceneuronalactivityinthecortex,hippocampusandbasalnuclei,whileneuronalactivityinthediencephalonandbrainstemwereonlyinfluencedbylong-termexercise.Acuteexercise-inducedchangesintheBDNFlevelswerepositivelycorrelatedwithchangesinthedensityofc-Fos+cells.Physicalexercisemayserveasanon-pharmaceuticalmeanstoprotectbrain,especiallythoseregionsrespondingtoexercisetraining. 中文摘要IAbstractIII致謝VFigureContentsVIIITableContentsIXAbbreviationXI.Introduction11.Exercise12.Neuronalactivity13.Brain-derivedneurotrophicfactor(BDNF)2II.ObjectiveandSpecificAims4III.MaterialsandMethods51.Animals52.Treadmillrunningprocedure53.Brainpreparation64.Immunohistochemistry75.Cellcounting76.Immunoblotting87.Statisticalanalysis9IV.Results101.Effectofsingle-boutacuteexerciseonneuronalactivityindifferentbrainregions102.Effectofone-monthlong-termexerciseonneuronalactivityindifferentbrainregions103.Changesofdensityofc-Fos+cells114.Comparingthechangesofdensityofc-Fos+cellsbetweensingle-boutacuteexerciseandone-monthlong-termexercise125.Effectofsingle-boutacuteexerciseonBDNFexpression126.Correlationbetweendensityofc-Fos+cellsandBDNFlevels13V.Discussion14VI.Conclusion18VII.References19 FigureContentsFigure1.Stereotaxicunitofmousebrain.26Figure2.Theprocedureofassigningabrainregionandcalculatethedensityofc-Fos+cells.27Figure3.Expressionpatternsofc-Fos+cellsbefore(Ctrl),immediatelyafter(E1h)andtwohoursafter(E1hS2h)1-htreadmillrunning.28Figure4.Effectofsingle-boutacuteexerciseondensityofc-Fos+cellsindifferentbrainregionsofmice.31Figure5.Effectofone-monthlong-termexerciseondensityofc-Fos+cellsindifferentbrainregionsofmice.34Figure6.Changesofdensityofc-Fos+cellsindifferentbrainregionsofmice.35Figure7.Effectofsingle-boutacuteexerciseonBDNFexpressioninthe8selectedbrainregions.37Figure8.Correlationbetweendensityofc-Fos+cellsandBDNFlevels.38Table1.Changesofdensityofc-Fos+cellsindifferentbrainregionsofmicethathavebeenexperiencedasingle-boutacuteexerciseand1-Mlong-termexercise………….39 AdkinsDL,BoychukJ,RempleMS,KleimJA(2006)Motortraininginducesexperience-specificpatternsofplasticityacrossmotorcortexandspinalcord.JApplPhysiol101:1776-1782.BaskinDS,MehlerWR,HosobuchiY,RichardsonDE,AdamsJE,FlitterMA(1986)Autopsyanalysisofthesafety,efficacyandcartographyofelectricalstimulationofthecentralgrayinhumans.BrainRes371:231-236.BerchtoldNC,ChinnG,ChouM,KesslakJP,CotmanCW(2005)Exerciseprimesamolecularmemoryforbrain-derivedneurotrophicfactorproteininductionintherathippocampus.Neuroscience133:853-861.BolamJP,HanleyJJ,BoothPAC,BevanMD(2000)Synapticorganisationofthebasalganglia.JAnat196:527-542.BramhamCR,MessaoudiE(2005)BDNFfunctioninadultsynapticplasticity:thesynapticconsolidationhypothesis.ProgNeurobiol76:99-125.ClarkPJ,BhattacharyaTK,MillerDS,RhodesJS(2011)Inductionofc-Fos,Zif268,andArcfromacuteboutsofvoluntarywheelrunninginnewandpre-existingadultmousehippocampalgranuleneurons.Neuroscience184:16-27.CotmanCW,BerchtoldNC(2002)Exercise:abehavioralinterventiontoenhancebrainhealthandplasticity.TrendsNeurosci25:295-301.DongM,WuY,FanY,XuM,ZhangJ(2006)c-fosmodulatesbrain-derivedneurotrophicfactormRNAexpressioninmousehippocampalCA3anddentategyrusneurons.NeurosciLett400:177-180.DumanCH,SchlesingerL,RussellDS,DumanRS(2008)Voluntaryexerciseproducesantidepressantandanxiolyticbehavioraleffectsinmice.BrainRes1199:148-158.EdlingY,Ingelman-SundbergM,SimiA(2007)Glutamateactivatesc-fosinglialcellsviaanovelmechanisminvolvingtheglutamatereceptorsubtypemGlu5andthetranscriptionalrepressorDREAM.Glia55:328-340.GaiddonC,LoefflerJP,LarmetY(1996)Brain-derivedneurotrophicfactorstimulatesAP-1andcyclicAMP-responsiveelementdependenttranscriptionalactivityincentralnervoussystemneurons.JNeurochem66:2279-2286.GeorgeP,KeithB.J.F(1998)Themousebraininstereotaxiccoordinates.GulfProfessionalPublishing.GottmannK,MittmannT,LessmannV(2009)BDNFsignalingintheformation,maturationandplasticityofglutamatergicandGABAergicsynapses.ExpBrainRes199:203-234.GuzowskiJF,TimlinJA,RoysamB,McNaughtonBL,WorleyPF,BarnesCA(2005)Mappingbehaviorallyrelevantneuralcircuitswithimmediate-earlygeneexpression.CurrOpinNeurobiol15:599-606.HerreroMT,BarciaC,NavarroJM(2002)Functionalanatomyofthalamusandbasalganglia.ChildsNervSyst18:386-404.HolschneiderDP,YangJ,GuoY,MaarekJM(2007)Reorganizationoffunctionalbrainmapsafterexercisetraining:importanceofcerebellar-thalamic-corticalpathway.BrainRes1184:96-107.HornEM,KramerJM,WaldropTG(2000)Developmentofhypoxia-inducedFosexpressioninratcaudalhypothalamicneurons.Neuroscience99:711-720.IchiyamaRM,GilbertAB,WaldropTG,IwamotoGA(2002)Changesintheexerciseactivationofdiencephalicandbrainstemcardiorespiratoryareasaftertraining.BrainRes947:225-233.ImamuraL,HasegawaH,KurashinaK,HamanishiA,TabuchiA,TsudaM(2000)Repressionofactivity-dependentc-fosandbrain-derivedneurotrophicfactormRNAexpressionbypyrethroidinsecticidesaccompanyingadecreaseinCa(2+)influxintoneurons.JPharmacolExpTher295:1175-1182.IwamotoGA,WappelSM,FoxGM,BuetowKA,WaldropTG(1996)Identificationofdiencephalicandbrainstemcardiorespiratoryareasactivatedduringexercise.BrainRes726:109-122.JeeYS,KoIG,SungYH,LeeJW,KimYS,KimSE,KimBK,SeoJH,ShinMS,LeeHH,ChoHJ,KimCJ(2008)Effectsoftreadmillexerciseonmemoryandc-Fosexpressioninthehippocampusoftheratswithintracerebroventricularinjectionofstreptozotocin.NeurosciLett443:188-192.JohnP.P,CedricX.B,FabioC(2015)Exercisephysiology.F.A.Daviscompany.JooJY,SchaukowitchK,FarbiakL,KilaruG,KimTK(2016)Stimulus-specificcombinatorialfunctionalityofneuronalc-fosenhancers.NatNeurosci19:75-83.KramerJM,PloweyED,BeattyJA,LittleHR,WaldropTG(2000)Hypothalamus,hypertension,andexercise.BrainResBull53:77-85.KuzniewskaB,RejmakE,MalikAR,JaworskiJ,KaczmarekL,KalitaK(2013)Brain-derivedneurotrophicfactorinducesmatrixmetalloproteinase9expressioninneuronsviatheserumresponsefactor/c-Fospathway.MolCellBiol33:2149-2162.LeeS,WilliamsonJ,LothmanEW,SzeleFG,ChesseletMF,VonHagenS,SapolskyRM,MattsonMP,ChristakosS(1997)EarlyinductionofmRNAforcalbindin-D28kandBDNFbutnotNT-3inrathippocampusafterkainicacidtreatment.BrainResMolBrainRes47:183-194.LeeTH,JangMH,ShinMC,LimBV,KimYP,KimH,ChoiHH,LeeKS,KimEH,KimCJ(2003)Dependenceofrathippocampalc-Fosexpressiononintensityanddurationofexercise.LifeSci72:1421-1436.LiJ,MitchellJH(2000)c-Fosexpressioninthemidbrainperiaqueductalgrayduringstaticmusclecontraction.AmJPhysiolHeartCircPhysiol279:H2986-H2993.MarsdenCD,ObesoJA(1994)ThefunctionsofthebasalgangliaandtheparadoxofstereotaxicsurgeryinParkinson'sdisease.Brain117:877-897.MayerDJ,WolfleTL,AkilH,CarderB,LiebeskindJC(1971)Analgesiafromelectricalstimulationinthebrainstemoftherat.Science174:1351-1354.MurrayPS,HolmesPV(2011)Anoverviewofbrain-derivedneurotrophicfactorandimplicationsforexcitotoxicvulnerabilityinthehippocampus.IntJPept2011:654085.NambuA(2004)Anewdynamicmodelofthecortico-basalganglialoop.ProgBrainRes143:461-466.NishijimaT,KawakamiM,KitaI(2013)Long-termexerciseisapotenttriggerforΔFosBinductioninthehippocampusalongthedorso-ventralaxis.PLoSOne8:e81245.NumakawaT,SuzukiS,KumamaruE,AdachiN,RichardsM,KunugiH(2010)BDNFfunctionandintracellularsignalinginneurons.HistolHistopathol25:237-258.NunezP,DiazB,PerillanC,VijandeM,ArguellesJ(2012)Progressivetrainingeffectsonneuronalhypothalamicactivationintherat.NeurosciLett517:113-117.OhiwaN,SaitoT,ChangH,NakamuraT,SoyaH(2006)Differentialresponsivenessofc-Fosexpressionintheratmedullaoblongatatodifferenttreadmillrunningspeeds.NeurosciRes54:124-132.ParkH,PooMM(2013)Neurotrophinregulationofneuralcircuitdevelopmentandfunction.NatRevNeurosci14:7-23.PerreyS(2013)Promotingmotorfunctionbyexercisingthebrain.BrainSci3:101-122.PorroCA,FrancescatoMP,CettoloV,DiamondME,BaraldiP,ZuianiC,BazzocchiM,diPramperoPE(1996)Primarymotorandsensorycortexactivationduringmotorperformanceandmotorimagery:afunctionalmagneticresonanceimagingstudy.JNeurosci16:7688-7698.RhodesJS,GarlandT.Jr,GammieSC(2003)Patternsofbrainactivityassociatedwithvariationinvoluntarywheel-runningbehavior.BehavNeurosci117:1243-1256.RosenfeldJP(1994)Interactingbrainstemcomponentsofopiate-activated,descending,pain-inhibitorysystems.NeurosciBiobehavRev18:403-409.SasakiK,GembaH(1984)Compensatorymotorfunctionofthesomatosensorycortexforthemotorcortextemporarilyimpairedbycoolinginthemonkey.ExpBrainRes55:60-68.SchoenfeldTJ,RadaP,PieruzziniPR,HsuehB,GouldE(2013)Physicalexercisepreventsstress-inducedactivationofgranuleneuronsandenhanceslocalinhibitorymechanismsinthedentategyrus.JNeurosci33:7770-7777.SmithY,BevanMD,ShinkE,BolamJP(1998)Microcircuitryofthedirectandindirectpathwaysofthebasalganglia.Neuroscience86:353-387.SnyderJS,RamchandP,RabbettS,RadikR,WojtowiczJM,CameronHA(2011)Septo-temporalgradientsofneurogenesisandactivityin13-month-oldrats.NeurobiolAging32:1149-1156.StröhleA(2009)Physicalactivity,exercise,depressionandanxietydisorders.JNeuralTransm116:777-784.VannSD,BrownMW,ErichsenJT,AggletonJP(2000)Fosimagingrevealsdifferentialpatternsofhippocampalandparahippocampalsubfieldactivationinratsinresponsetodifferentspatialmemorytests.JNeurosci20:2711-2718.VossMW,VivarC,KramerAF,vanPraagH(2013)Bridginganimalandhumanmodelsofexercise-inducedbrainplasticity.TrendsCognSci17:525-544.WilliamsCA,HoltsclawLI,ChivertonJA(1992)Releaseofimmunoreactiveenkephalinergicsubstancesintheperiaqueductalgreyofthecatduringfatiguingisometriccontractions.NeurosciLett139:19-23.WrannCD,WhiteJP,SalogiannnisJ,Laznik-BogoslavskiD,WuJ,MaD,LinJD,GreenbergME,SpiegelmanBM(2013)ExerciseinduceshippocampalBDNFthroughaPGC-1α/FNDC5pathway.CellMetab18:649-659.WuCW,ChangYT,YuL,ChenHI,JenCJ,WuSY,LoCP,KuoYM(2008)Exerciseenhancestheproliferationofneuralstemcellsandneuritegrowthandsurvivalofneuronalprogenitorcellsindentategyrusofmiddle-agedmice.JApplPhysiol105:1585-1594.WuSY,WangTF,YuL,JenCJ,ChuangJI,WuFS,WuCW,KuoYM(2011)Runningexerciseprotectsthesubstantianigradopaminergicneuronsagainstinflammation-induceddegenerationviatheactivationofBDNFsignalingpathway.BrainBehavImmun25:135-146. 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