Pin-Ju Chueh | Institute of Biomedical Sciences - 生物醫學研究所

Pin-Ju Chueh. Professor. PhD, Purdue University. Room 1127, 11th Floor, Bioscience Building/Tumor Biochemical Mechanism Research Laboratory. Skiptomaincontent Pin-JuChueh Pin-JuChueh Professor PhD,PurdueUniversity Room1127,11thFloor,BioscienceBuilding/TumorBiochemicalMechanismResearchLaboratory (04)22840896#120 [email protected] Researchareas 我們在1995年美國國家科學院院刊(TheProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,PNAS)中，首度發表辣椒素有效的抑制tNOX腫瘤蛋白的活性，並發現這樣的活性被抑制後，導致腫瘤細胞的凋亡及細胞生長的降低；而辣椒素對非癌化的細胞則無明顯的影響。

tNOX腫瘤蛋白為一NADH氧化酶酵素，其活性及蛋白表現與腫瘤細胞的生長與存活能力有重要的關係，我們也在癌症病人的血清之中偵測到tNOX腫瘤蛋白的活性及蛋白表現，但在非癌化的細胞或非癌症病人的血清之中，則只有相對低量的tNOX蛋白。

後續以RNA干擾技術的方式，將基因靜默進而達到使tNOX蛋白質的表現量降低，我們發現tNOX蛋白表現量與細胞的增生與細胞移動能力有正向的關係；反之，當tNOX蛋白被刺激增加或大量表現時，細胞的侵犯性也隨之增加並伴隨著EMT使細胞驅向癌化。

目前我們認為tNOX腫瘤蛋白之所以可以參與細胞內許多功能的調控，主要是因為其NADH氧化酶的酵素能力，影響細胞內NAD+與NADH的濃度比率，進而調節去乙烯基酶SIRT1的酵素能力，而SIRT1將其他蛋白質去乙烯基後所可影響細胞能量代謝及老化等。

目前，實驗室的研究領域主要可以分成下列幾大方向： 1.以細胞株模式及動物實驗探討tNOX腫瘤蛋白及其所調控的SIRT1在癌症發展所扮演的角色，如細胞週期、細胞死亡及細胞移動等生物功能之調控。

2.tNOX腫瘤蛋白SIRT1之間相互的調控機制。

  3.抑癌藥物所引發細胞凋亡及細胞自噬機制之探討。

4.奈米物質的細胞毒性及其引發毒性機制之探討。

Coursesoffering 上學期-醫用生物技術(一)(2學分) 上學期-細胞死亡之分子機制特論(2學分) 上學期-氧化還原酶蛋白功能特論(2學分) 上學期-蛋白結構及功能(2學分) 下學期-細胞増生之分子機制特論(2學分) 下學期-醫用生物技術(二)(2學分) 下學期-氧化酶蛋白與疾病特論(2學分) 全學年-NADH氧化酶蛋白功能分析研究(6學分) WorkExperience 教授-國立中興大學生物醫學研究所(2/2013-迄今) 國際處學術交流組組長-國立中興大學(9/2010-5/2014) 副教授-國立中興大學生物醫學研究所(2/2009-1/2013) 助理教授-國立中興大學生物醫學研究所-(2/2005-1/2009)  助理教授-中山醫學大學生命科學系-(9/2003-2/2005)  助理研究員-美國普渡大學藥物化學暨分子藥理研究所-(PurdueUniversity)(10/2001-8/2003) 博士後研究員-美國普渡大學藥物化學暨分子藥理研究所(PurdueUniversity)(3/1998-9/2001) 博士-美國普渡大學-(1/1994-12/1997) 碩士-國立中興大學化學研究所(9/1989-6/1991) 學士-國立中興大學化學系(9/1986-6/1989) Award 擔任國際學術期刊評審委員:SCI期刊審查 AdvancedHealthcareMaterials BiochemistryandCellBiology Biochimie BioresourceTechnology BiotechnologyAdvances CancerBiology&Therapy CancerImmunologyImmunotherapy Chemosphere ChineseJournalofPhysiology ColloidsandSurfacesB:Biointerfaces  EnzymeandMicrobialTechnology ExperimentalCellResearch InternationalJournalofMolecularSciences InvestigationalNewDrugs JournalofAgriculturalandFoodChemistry JournalofCellularPhysiology JournalofDietarySupplements JournalofHazardousMaterials JournalofNanoparticleResearch MolecularandCellularBiochemistry MolecularMembraneBiology Nanomedicine PlosOne ProcessBiochemistry ToxicologyLetters AcademicWorks A.期刊論文 Lin,M.H.,Lee,Y.H.,Cheng,H.L.,Chen,H.Y.,Jhuang,F.H., Chueh,P.J.*(2016)CapsaicinInhibitsMultipleBladderCancerCellPhenotypesbyInhibitingTumor-AssociatedNADHOxidase(tNOX)andSirtuin1(SIRT1).Molecules21(7):pii:E849 HungYenChen,Yi-HuiLee,HueiYuChen,ChiaAnYeh,PinJuChueh,andYi-MeiJ.Lin*(2016)Capsaicin-inhibitedaggressivephenotypesisthroughdownregulationoftumor-associatedNADHoxidase(tNOX)byPOUdomaintranscriptionfactorBRN2.Molecules21(6):pii:E733 Wu,C.C.,Cheng,C.H.,Lee,Y.H.,Chang,I.L.,Chen,H.Y.,Hsieh,C.P., Chueh,P.J.* (2016)UrsolicAcidTriggersApoptosisinHumanOsteosarcomaCellsviaCaspaseActivationandtheERK1/2MAPKPathway.JAgricFoodChem.64(21):4220-6 Cheng,H.L., Lee,Y.H., Yuan,T.M., Chen,S.W., Chueh,P.J.*(2016)Updateonatumor-associatedNADHoxidaseingastriccancercellgrowth.WorldJGastroenterol.22(10):2900-5  Tikhomirov,A.S.,Shchekotikhin,A.E.,Lee,Y.H.,Chen,Y.A.,Yeh,C.A.,Tatarskiy,V.V.Jr,Dezhenkova,L.G.,Glazunova,V.A.,Balzarini,J.,Shtil,A.A.,Preobrazhenskaya,M.N., Chueh,P.J.*(2015)SynthesisandCharacterizationof4,11-Diaminoanthra[2,3-b]furan-5,10-diones:TumorCellApoptosisthroughtNOX-ModulatedNAD(+)/NADHRatioandSIRT1.JMedChem.58(24):9522-34.  Lee,Y.H.,Chen,H.Y.,Su,L.J.,Chueh,P.J.*(2015)Sirtuin1(SIRT1)deacetylaseactivityandNAD+/NADHratioareimperativeforcapsaicin-mediatedprogrammedcelldeath.JAgricFoodChem.63,7361-70. Liang,R.Y.,Tu,H.F.,Tan,X.T.,Yeh,Y.S.,Chueh,P.J.,Chuang,S.M.*(2015)Agenesignatureforgoldnanoparticleexposedhumancelllines.ToxicolRes.4,365-375. Yuan,T.M.,Liang,R.Y.,Chueh,P.J.,Chuang,S.M.*(2015)RoleofribophorinIIintheresponsetoanticancerdrugsingastriccancercelllines.OncolLett.9,1861-1868. Kumar,K.J.,Vani,M.G.,Chueh,P.J.,Mau,J.L.,Wang,S.Y.*(2015)AntrodinCinhibitsepithelial-to-mesenchymaltransitionandmetastasisofbreastcancercellsviasuppressionofSmad2/3andβ-cateninsignalingpathways.PLoSOne10(2):e0117111. Chueh,P.J.,Liang,R.Y.,Lee,Y.H.,Zeng,Z.M.,Chuang,S.M.*(2014)Differentialcytotoxiceffectsofgoldnanoparticlesindifferentmammaliancelllines.JHazardMater.264,303-312. ChuangSM,LeeYH,LiangRY,RoamGD,ZengZM,TuHF,WangSK,Chueh,P.J.*(2013)Extensiveevaluationsofthecytotoxiceffectsofgoldnanoparticles.BiochimBiophysActa.1830(10):4960-73. Su,Y.C.,Lin,Y.H.,Zeng,Z.H.,Shao,K.N.,Chueh,P.J.*(2012)Chemotherapeuticagentsenhancecellmigrationandepithelial-to-mesenchymaltransitionthroughtransientup-regulationoftNOX(ENOX2)protein.BiochimicaetBiophysicaActa-GeneralSubjects1820(11):1744-52. Zeng,Z.H.,Chuang,S.M.,Chang,T.C.,Hong,C.W.,Chou,J.C.,Yang,J.J.,Chueh,P.J.*,“(2012)Phosphorylationofserine-504oftNOX(ENOX2)modulatescellproliferationandmigrationincancercells.,”Exp.CellRes.,vol.318,pp.1759-1766.,072012. Liu,N.C.,Hsieh,P.F.,Hsieh,M.K.,Zeng,Z.M.,Cheng,H.L.,Liao,J.W.,Chueh,P.J.*,“(2012)Capsaicin-MediatedtNOX(ENOX2)Up-regulationEnhancesCellProliferationandMigrationinVitroandinVivo.,”JAgricFoodChem.,vol.60.,pp.2758-2765,012012. Wang,H.M.,Chuang,S.M.,Su,Y.C.,Li,Y.H.,Chueh,P.J.*,“(2011)Down-RegulationofTumor-AssociatedNADHOxidase,tNOX(ENOX2),EnhancesCapsaicin-InducedInhibitionofGastricCancerCellGrowth.,”CellBiochemBiophys.,vol.61,pp.355-366,012011. 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Morré,D.J.,Chueh,P.J.,Yagiz,K.,Balicki,A.,Kim,C.,andMorré,D.M.,“(2007)ECTO-NOXtargetfortheanticancerisoflavenephenoxodiol,”OncologyResearch16(7),pp.299-312,012007. Chen,C.F.,Huang,S.,Liu,S.C.andChueh,P.J.*,“(2006)EffectofpolyclonalantiseratorecombinanttNOXproteinonthegrowthoftransformedcells,”BioFactors,vol.28(2),pp.119-133,012006. Chueh,P.J.*,Wu,L.Y.,Morré,D.M.andMorré,D.J.,“(2004)tNOXisbothnecessaryandsufficientasacellulartargetfortheanticanceractionsofcapsaicinandthegreenteacatechin(-)-epigallocatechin-3-gallate,”BioFactors,vol.20(4),pp.249-263,012004. Huang,C.Y.,Chueh,P.J.*,Tseng,C.T.,Liu,K.Y.,Tsai,H.Y.,Kuo,W.W.,Chou,M.Y.,andYang,J.J.,“(2004)ZAKre-programsatrialnatriureticfactorexpressionandinduceshypertrophicgrowthinH9c2cardiomyoblastcells,”BiochemBiophysResCommun.,vol.324(3),pp.973-980,012004. 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