pAAV-cFos-tTA-pA (Plasmid #66794) - Addgene

Plasmid pAAV-cFos-tTA-pA from Dr. William Wisden's lab contains the insert cfos promoter linked to the tet activator gene and is published in Nat Neurosci. Morethan20requests Morethan50requests Morethan100requests Image:IllustratedplasmidmapinPNGformat GenBankFile:Plasmidsequenceandannotations.Usetexteditororplasmidmappingsoftwaretoviewsequence. SnapGeneFile:PlasmidsequenceandSnapGeneenhancedannotations.UsewithSnapGenesoftwareorthefreeViewertovisualizeadditionaldataandalignothersequences. × Close AddgeneFullSequenceMapforpAAV-cFos-tTA-pA DownloadFile Image GenBank SnapGene Viewallsequences Close × Close MapImageforpAAV-cFos-tTA-pA Mapuploadedbythedepositor. DownloadImage Viewallsequences Close × Close MapImageforpAAV-cFos-tTA-pA Mapuploadedbythedepositor. DownloadImage Viewallsequences Close Skiptomaincontent Browse WilliamWisden Zhangetal pAAV-cFos-tTA-pA pAAV-cFos-tTA-pA (Plasmid #66794) Print Enlarge Viewallsequences Enlarge Download Enlarge Download Purpose ExpressesTetActivatorproteinunderthecontrolofthec-fospromoterforuseinTetTaging DepositingLab WilliamWisden Publication Zhangetal NatNeurosci.2015Apr;18(4):553-61.doi:10.1038/nn.3957.Epub2015Feb23. ( Howtocite) SequenceInformation Sequences(3) Ordering Item Catalog# Description Quantity Price(USD) Plasmid 66794 Standardformat:Plasmidsentinbacteriaasagarstab 1 $75 AddtoCart Thismaterialisavailabletoacademicsandnonprofitsonly. Backbone Vectorbackbone pUC (SearchVectorDatabase) Backbonesize w/oinsert (bp) 3600 Totalvectorsize(bp) 6673 Vectortype MammalianExpression,AAV GrowthinBacteria BacterialResistance(s) Ampicillin GrowthTemperature 37°C GrowthStrain(s) NEBStable Copynumber HighCopy Gene/Insert Gene/Insertname cfospromoterlinkedtothetetactivatorgene Species Synthetic InsertSize(bp) 1945 Tag /FusionProtein no CloningInformation Cloningmethod RestrictionEnzyme 5′cloningsite PacI (unknownifdestroyed) 3′cloningsite NotI (unknownifdestroyed) 5′sequencingprimer GACCATCTCCGAAATCCTACACG 3′sequencingprimer ACAAAGGCATTAAAGCAGCGTATCCACAT (CommonSequencingPrimers) ResourceInformation Aportionofthisplasmidwasderivedfromaplasmidmadeby cfos-tTAwastakenfromAddgeneplasmidID#:34856 ArticlesCitingthisPlasmid 3References TermsandLicenses Academic/NonprofitTerms UBMTA IndustryTerms NotAvailabletoIndustry Trademarks: Zeocin®isanInvivoGentrademark. Howtocitethisplasmid (Backtotop) Theseplasmidswerecreatedbyyourcolleagues.Pleaseacknowledgethe PrincipalInvestigator,citethearticleinwhichtheplasmidsweredescribed, andincludeAddgeneintheMaterialsandMethodsofyourfuturepublications. ForyourMaterials&Methodssection: pAAV-cFos-tTA-pA wasagiftfrom WilliamWisden (Addgeneplasmid#66794;http://n2t.net/addgene:66794;RRID:Addgene_66794) ForyourReferencessection: Neuronalensemblessufficientforrecoverysleepandthesedativeactionsofalpha2adrenergicagonists. ZhangZ,FerrettiV,GuntanI,MoroA,SteinbergEA,YeZ,ZechariaAY,YuX,VyssotskiAL,BrickleySG,YustosR,PillidgeZE,HardingEC,WisdenW,FranksNP. NatNeurosci.2015Apr;18(4):553-61.doi:10.1038/nn.3957.Epub2015Feb23. 10.1038/nn.3957 PubMed25706476 Relateditems: Fromthisarticle WilliamWisdenLabPlasmids Adeno-associatedViral(AAV)plasmids Commonlyrequestedwith: pAAV-PTRE-tight-hM3Dq-mCherry pAAV-TRE-EGFP pK170.AAV-TRE-Cre-WPRE (Supernova) pAAV-RAM-d2TTA::TRE-EGFP-WPREpA pOTTC476-pAAVc-foseYFP Mapuploadedbythedepositor. Mapuploadedbythedepositor.