mMESSAGE mMACHINE™ T7 Transcription Kit

AM1344. Unit Size. 25 reactions. Price (USD). Price: 556.00. Your Price: Sign In. Availability. -. Quantity. Save to list. Add to cart ... PopularApplications&TechniquesShopAllProductsServicesSupport Signin QuickOrder     Search ThermoFisherScientific SearchAll Search HomeShopAllProductsBiochemicalReagentsTranscriptionandTranslationReagentsmMESSAGEmMACHINE™T7TranscriptionKitInvitrogen™mMESSAGEmMACHINE™T7TranscriptionKitCatalognumber:AM1344Relatedapplications:InVitroTranscriptionTechnicalSupportCustomerServiceInvitrogen™mMESSAGEmMACHINE™T7TranscriptionKitCatalognumber:AM1344Relatedapplications:InVitroTranscriptionTechnicalSupportCustomerServiceCatalogNumberAM1344UnitSize25reactionsPrice(SGD)RequestAQuoteCatalogNumberUnitSizePrice(SGD)AM134425reactionsRequestAQuoteProductOverviewFiguresVideosDocumentsFAQCitations&ReferencesAdditionalInformationRecommendationsRecommendationsmMESSAGEmMACHINE™kitsaredesignedfortheinvitrosynthesisoflargeamountsofcappedRNA.CappedRNAmimicsmosteukaryoticmRNAsfoundinvivo,becauseithasa7-methylguanosinecapstructureatthe5'end.mMESSAGEmMACHINE™kitreactionsincludecapanalog[m7G(5')ppp(5')G]inanultrahigh-yieldtranscriptionreaction.Thecapanalogisincorporatedonlyasthefirstor5'terminalGofthetranscriptbecauseitsstructureprecludesitsincorporationatanyotherpositionintheRNAmolecule.HowmMESSAGEmMACHINE™kitsworkmMESSAGEmMACHINE™kitshaveasimplifiedreactionformatinwhichallfourribonucleotidesandcapanalogaremixedinasinglesolution.Thecapanalog:GTPratioofthissolutionis4:1,whichisoptimalformaximizingbothRNAyieldandtheproportionofcappedtranscripts.mMESSAGEmMACHINE™kitsareidealfortheroutinesynthesisofcappedRNAsforoocytemicroinjection,invitrotranslation,transfection,andotherapplications.ThehighyieldsareachievedbyoptimizingreactionconditionsforRNAsynthesisinthepresenceofhighnucleotideconcentrations.Inaddition,theRNAsynthesizedisprotectedfromdegradationbyanycontaminatingribonucleasesthatmaybepresentwithRNaseinhibitor—acomponentoftheEnzymeMix.UsingmMESSAGEmMACHINE™KitsIna20µLreactionduringa2hourincubation,mMESSAGEmMACHINE™HighYieldCappedRNATranscriptionwillyieldlargemassamountsofcappedRNA.Upto10–50timestheyieldobtainedwithconventionalinvitrotranscriptionreactions.TheratioofcapanalogtoGTPhasbeenoptimizedtoallowthebestcompromisebetweenyield(15–35µg)andproportionoftranscriptsthatarecapped(80%).mMESSAGEmMACHINE™KitsalsocontainaLiClprecipitationsolutionthatisefficientforseparatingproteinsandunincorporatednucleotides(includingfreecapanalog)fromthecappedRNA,allowinganincreasedefficiencyoftranslation.mMESSAGEmMACHINE™kitsareonlyoptimizedforusewiththepolymerasesincludedinthekit.Usingadifferentpolymerasemayresultinlowyields.ThemMESSAGEmMACHINE™kitscontainallthebuffersandreagentsnecessaryfor25transcriptionreactions.Usingthecontroltemplatesuppliedwiththekits(Xenopuselongationfactor1α,pTRIXef),eachmMESSAGEmMACHINE™reactionwillyieldapproximately20–30µgofRNAusingT3orT7RNApolymerase,orabout15–25µgRNAusingSP6RNApolymerase.ForResearchUseOnly.Notforuseindiagnosticprocedures.SpecificationsFinalProductTypesyntheticmRNA,mRNAFormatKitSampleTypemRNA,syntheticmRNAForUseWith(Application)InVitroTranscription,syntheticmRNA,mRNA,transfection,LNPs,GENEart,linearizedDNAplasmid,delivery,PCRproductsNo.ofReactions25ReactionsProductLineAmbion™,mMESSAGEmMACHINE™ProductTypeT7InVitroTranscriptionkitQuantity25ReactionsPromoterT7ShippingConditionDryIceYield20-30µgStartingMaterialPCRProducts,LinearizedDNAplasmidContents&StorageT7EnzymeMix,10XReactionBuffer2XNTP⁄CAPSolution,GTPSolution,pTRI-Xef,TURBODNase,AmmoniumAcetateStopSolution,LithiumChloridePrecipitationSolution,andGelLoadingBufferIIareallstoredat–20°C.Nuclease-freeWatermaybestoredatanytemperature. 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