A protocol for mosaic analysis with a repressible cell marker ...

Mosaic analysis with a repressible cell marker (MARCM) is a genetic technique used in Drosophila to label single cells or multiple cells ... Skiptomaincontent Thankyouforvisitingnature.com.YouareusingabrowserversionwithlimitedsupportforCSS.Toobtain thebestexperience,werecommendyouuseamoreuptodatebrowser(orturnoffcompatibilitymodein InternetExplorer).Inthemeantime,toensurecontinuedsupport,wearedisplayingthesitewithoutstyles andJavaScript. Advertisement nature natureprotocols protocols article Thisarticlehasbeenupdated AbstractMosaicanalysiswitharepressiblecellmarker(MARCM)isagenetictechniqueusedinDrosophilatolabelsinglecellsormultiplecellssharingasingleprogenitor.Labeledhomozygousmutantcellscanbegeneratedinanotherwiseunlabeledheterozygousanimal.Mutantorwild-typelabeledcellscanalsobemadetoexpressoneormoretransgenes.MajorapplicationsofMARCMinclude(i)lineageanalysis,(ii)investigatinggenefunctioninsingleorsmallpopulationsofcellsand(iii)neuronalcircuittracing.OurlaboratoryusesMARCMprimarilytolabelandgeneticallymanipulateneurons;however,thisprotocolcanbeadaptedtoanycellofinterest.TheprotocolinvolvesgeneratingtwoflystockswiththenecessarygeneticelementsforMARCManalysisandsubsequentlygeneratingMARCMclones.Labeledclonescanbefollowedinliveandfixedtissuesforhigh-resolutionanalysisofwild-typeorgeneticallymanipulatedcells.NOTE:InthePDFversionofthisarticleinitiallypublishedonline,thefirst“FRT”andthe“Mutation”labelsinFigure1bweretransposed.InboththePDFandHTMLversions,“mutant”wasomittedfromthelabelontheright,whichshouldread“Labeledhomozygousmutantdaughtercell”.Thefigurehasbeencorrectedinallversionsofthearticle. Yourinstitutedoesnothaveaccesstothisarticle Relevantarticles OpenAccessarticlescitingthisarticle. DrosophilaGrafregulatesmushroombodyβ-axonextensionandolfactorylong-termmemory SungdaeKim,JoohyungKim … SeungbokLee MolecularBrain OpenAccess 23April2021 RoleofArmadillorepeat2andkinesin-IImotorsubunitKlp64DforwinglesssignalinginDrosophila LinhThuongVuong,Jong-HoonWon … Kwang-WookChoi ScientificReports OpenAccess 17August2020 Ciao1interactswithCrumbsandXpdtoregulateorgangrowthinDrosophila JeanJung,EunbyulYeom & Kwang-WookChoi CellDeath&Disease OpenAccess 13May2020 Accessoptions Accessthroughyourinstitution Changeinstitution Buyorsubscribe SubscribetoJournalGetfulljournalaccessfor1year92,52€only7,71€perissueSubscribeAllpricesareNETprices.VATwillbeaddedlaterinthecheckout.Taxcalculationwillbefinalisedduringcheckout.BuyarticleGettimelimitedorfullarticleaccessonReadCube.$32.00BuyAllpricesareNETprices. Additionalaccessoptions: Login Learnaboutinstitutionalsubscriptions Figure1:SchematicrepresentationoftheGAL4–UASsystemwithGAL80andtheMARCMgeneticsystem.Figure2:CrossbetweenaMARCM-readyandaFRT,mutantflyforMARCManalysis.Figure3:MARCMexampleinwhichthebirthorderofprojectionneuronspredictstheirdendritic-projectionpattern.Figure4:MARCMexamplewithhigh-resolutionaxonphenotypesinsingle-cellmutantandsingle-cellrescueexperiments. 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RightsandpermissionsReprintsandPermissionsAboutthisarticleCitethisarticleWu,J.,Luo,L.Aprotocolformosaicanalysiswitharepressiblecellmarker(MARCM)inDrosophila. NatProtoc1,2583–2589(2006).https://doi.org/10.1038/nprot.2006.320DownloadcitationPublished:11January2007IssueDate:December2006DOI:https://doi.org/10.1038/nprot.2006.320SharethisarticleAnyoneyousharethefollowinglinkwithwillbeabletoreadthiscontent:GetshareablelinkSorry,ashareablelinkisnotcurrentlyavailableforthisarticle.Copytoclipboard ProvidedbytheSpringerNatureSharedItcontent-sharinginitiative Furtherreading DrosophilaGrafregulatesmushroombodyβ-axonextensionandolfactorylong-termmemory SungdaeKim JoohyungKim SeungbokLee MolecularBrain(2021) RoleofArmadillorepeat2andkinesin-IImotorsubunitKlp64DforwinglesssignalinginDrosophila LinhThuongVuong Jong-HoonWon Kwang-WookChoi ScientificReports(2020) Ciao1interactswithCrumbsandXpdtoregulateorgangrowthinDrosophila JeanJung EunbyulYeom Kwang-WookChoi CellDeath&Disease(2020) Theprion-likedomainofDrosophilaImppromotesaxonaltransportofRNPgranulesinvivo JeshleeVijayakumar CharlènePerrois FlorenceBesse NatureCommunications(2019) CommentsBysubmittingacommentyouagreetoabidebyourTermsandCommunityGuidelines.Ifyoufindsomethingabusiveorthatdoesnotcomplywithourtermsorguidelinespleaseflagitasinappropriate. 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