Cell Line Generation - Texcell
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Cell Line Generation · Transfection of recombinant plasmid DNA into mammalian cell lines via lipofectamine or PEI, or transduction of a lentiviral vector · Stable ... Texcell/ResearchCellBanking/CellLineGeneration Texcell’sCellCultureteamhasnearly25yearsofexperienceusingcell-basedsystemsforproteinexpression. Akeyfactorincontrollingproductioncostsofbiopharmaceuticalproteinsistherapiddevelopmentofrecombinantcelllinesthatproducehighlevelsofexpressedproteinproducts. Tohelpfacilitateachievingthisgoal,ourteamoffersthefollowingimportantcoreservicestoexpeditethedevelopmentprocessattheR&Dlevel: TransfectionofrecombinantplasmidDNAintomammaliancelllinesvialipofectamineorPEI,ortransductionofalentiviralvector Stablecellselectionusingappropriateselectiveagents(G418,hygromycin,puromycin,etc.) Single-cellplatingin96-wellplateorcloningbylimitingdilution CellclonevalidationbyELISAorqPCR Cryopreservationofthehigh-levelexpressercelllineclonesforfutureuse Preparationof aResearchCellBank(RCB)fromselectedclonalcelllines Usingtheaboveapproaches,aneffectiveselectionstrategycanbedesignedtoidentifymonoclonalcelllinesthatshouldstablyexpresshighlevelsofrecombinantproteinsinculture;thesecellswillthenbeideallysuitedfortransfertoourGMPCellBankingpriortolarge-scaleproductionofproteinsinstandardfed-batchfermentationprocesses. Biosafety RecombinantProteinsormAbs CellIdentity CellStability ViralTesting Sterility MycoplasmaTesting PyrogenicityTesting TransmissionElectronMicroscopy HostCellResiduals CellTherapy CellIdentity MycoplasmaTesting PyrogenicityTesting PotencyAssays TransmissionElectronMicroscopy Anti-DrugAntibodies GeneTherapy CellIdentity Sterility MycoplasmaTesting ViralTesting Tumorigenicity PyrogenicityTesting VectorInfectivityAssays PotencyAssays ViralVaccines Sterility MycoplasmaTesting ViralTesting TransmissionElectronMicroscopy Tumorigenicity PyrogenicityTesting VectorInfectivityAssays HumanorAnimaloriginProducts Sterility MycoplasmaTesting ViralTesting BatchRelease ViralClearance VirusPanel StudyTierstoConsider LevelsofService PrionClearanceStudies GMPCellBanking CellIdentity Sterility MycoplasmaTesting ManufacturingMCB/WCB ResearchCellBanking CellLineGeneration CellLineExpansion ClonalSelection ResearchCellBanking MedicalDevices BioAssaysinvitro ReporterGeneAssay BiomarkersExpression CytokinesExpression PotencyAssay BioAssaysinvivo CellProliferation Biodistribution Immunogenicity PotencyAssay Pharmacokinetic Tumorigenicity Toxicology Thiswebsiteusescookiestoimproveyourexperience.We'llassumeyou'reokwiththis,butyoucanopt-outifyouwish.AcceptRejectReadMorePrivacy&CookiesPolicy Close PrivacyOverview Thiswebsiteusescookiestoimproveyourexperiencewhileyounavigatethroughthewebsite.Outofthese,thecookiesthatarecategorizedasnecessaryarestoredonyourbrowserastheyareessentialfortheworkingofbasicfunctionalitiesofthewebsite.Wealsousethird-partycookiesthathelpusanalyzeandunderstandhowyouusethiswebsite.Thesecookieswillbestoredinyourbrowseronlywithyourconsent.Youalsohavetheoptiontoopt-outofthesecookies.Butoptingoutofsomeofthesecookiesmayaffectyourbrowsingexperience. Necessary Necessary AlwaysEnabled Necessarycookiesareabsolutelyessentialforthewebsitetofunctionproperly.Thiscategoryonlyincludescookiesthatensuresbasicfunctionalitiesandsecurityfeaturesofthewebsite.Thesecookiesdonotstoreanypersonalinformation. Non-necessary Non-necessary Anycookiesthatmaynotbeparticularlynecessaryforthewebsitetofunctionandisusedspecificallytocollectuserpersonaldataviaanalytics,ads,otherembeddedcontentsaretermedasnon-necessarycookies.Itismandatorytoprocureuserconsentpriortorunningthesecookiesonyourwebsite. SAVE&ACCEPT
延伸文章資訊
- 1Guideline for Generation of Stable Cell Lines
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- 2Generation of Stable Expression Mammalian Cell Lines Using ...
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- 3Generate Stable Cell Lines With Chemical Transfection
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- 4Cell Line Generation - Texcell
Cell Line Generation · Transfection of recombinant plasmid DNA into mammalian cell lines via lipo...
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Large panels of comprehensively characterized human cancer models, including the Cancer Cell Line...