pcDNA5/FRT/TO GFP (Plasmid #19444) - Addgene

Plasmid pcDNA5/FRT/TO GFP from Dr. Harm Kampinga's lab contains the insert GFP and is published in Cell Stress Chaperones. 2008 Aug 7. ():. Morethan20requests Morethan50requests Morethan100requests Image:IllustratedplasmidmapinPNGformat GenBankFile:Plasmidsequenceandannotations.Usetexteditororplasmidmappingsoftwaretoviewsequence. SnapGeneFile:PlasmidsequenceandSnapGeneenhancedannotations.UsewithSnapGenesoftwareorthefreeViewertovisualizeadditionaldataandalignothersequences. × Close AddgeneFullSequenceMapforpcDNA5/FRT/TOGFP DownloadFile Image GenBank SnapGene Viewallsequences Close × Close DepositorFullSequenceMapforpcDNA5/FRT/TOGFP DownloadFile Image GenBank SnapGene Viewallsequences Close Skiptomaincontent Browse HarmKampinga Hagemanetal pcDNA5/FRT/TOGFP pcDNA5/FRT/TOGFP (Plasmid #19444) Print Enlarge Viewallsequences Enlarge Viewallsequences DepositingLab HarmKampinga Publication Hagemanetal CellStressChaperones.2008Aug7.():. ( Howtocite) SequenceInformation Sequences(3) Ordering Item Catalog# Description Quantity Price(USD) Plasmid 19444 Standardformat:Plasmidsentinbacteriaasagarstab 1 $75 AddtoCart Thismaterialisavailabletoacademicsandnonprofitsonly. Backbone Vectorbackbone pcDNA5/FRT/TO (SearchVectorDatabase) Backbonemanufacturer Invitrogen Backbonesize w/oinsert (bp) 5137 Vectortype MammalianExpression Selectablemarkers Hygromycin GrowthinBacteria BacterialResistance(s) Ampicillin GrowthTemperature 37°C GrowthStrain(s) DH5alpha Copynumber HighCopy Gene/Insert Gene/Insertname GFP Species Aequoreavictoria InsertSize(bp) 872 CloningInformation Cloningmethod RestrictionEnzyme 5′cloningsite HindIII (notdestroyed) 3′cloningsite BamHI (notdestroyed) 5′sequencingprimer LNCX 3′sequencingprimer BGH-rev (CommonSequencingPrimers) ResourceInformation ArticlesCitingthisPlasmid 3References TermsandLicenses Academic/NonprofitTerms UBMTA AncillaryAgreementforPlasmidsContainingFPMaterials IndustryTerms NotAvailabletoIndustry Trademarks: Zeocin®isanInvivoGentrademark. DepositorComments Disclaimer: ForcloningthevariousHSPgenes,theStratagenereferenceRNAforcDNAsynthesiswasusedandgeneswereamplifiedfromthismixturethatoriginatesfrom10differentcelllinesfromdifferentsomaticoriginandultimatelycomingfrom10differentindividuals.ThisstrategywasusedtomaximizethechanceforthepresenceofindividualHSPtranscripts.Usersmustbeawarethatthismixturethusrepresents20completehaploidgenomes,meaningthatforeachindividualgenetheremightbepolymorphismsthatcouldpotentiallyhavefunctionalconsequences.Thelibrarywassequenceverifiedonlyforthepresenceofthecorrectgene. Howtocitethisplasmid (Backtotop) Theseplasmidswerecreatedbyyourcolleagues.Pleaseacknowledgethe PrincipalInvestigator,citethearticleinwhichtheplasmidsweredescribed, andincludeAddgeneintheMaterialsandMethodsofyourfuturepublications. ForyourMaterials&Methodssection: pcDNA5/FRT/TOGFP wasagiftfrom HarmKampinga (Addgeneplasmid#19444;http://n2t.net/addgene:19444;RRID:Addgene_19444) ForyourReferencessection: ComputationalanalysisofthehumanHSPH/HSPA/DNAJfamilyandcloningofahumanHSPH/HSPA/DNAJexpressionlibrary. HagemanJ,KampingaHH. CellStressChaperones.2008Aug7.():. 10.1007/s12192-008-0060-2 PubMed18686016 Relateditems: Fromthisarticle HarmKampingaLabPlasmids Commonlyrequestedwith: pCAG-Flpe pcDNA5/FRT/TO-Venus-Flag(865) pcDNA5/FRT/TOV5 pcDNA5/FRT/TOHIS pcDNA5/FRT/TO-neo(1795)