LAMP amplificationlamp檢測台灣lamp原理loop-mediated isothermal amplificationLAMP PCRlamp pcr原理lamp檢測lamp pcr比較

Design primers for Loop-Mediated Isothermal Amplification

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LAMP Designer designs efficient primers for Loop-Mediated Isothermal Amplification assays, that amplify DNA and RNA sequences at isothermal conditions, ... Togglenavigation HOME COMPANY PRODUCTS ORDERING SUPPORT BLOG Home>>Products>>LAMPDesigner>>Overview LAMPDesigner DesignPrimersforLoopMediatedIsothermalAmplification LAMPDesignerdesignsefficientprimersforLoop-MediatedIsothermalAmplificationassays,thatamplifyDNAandRNAsequencesatisothermalconditions,eliminatingthenecessityofaPCRsetup.Thetechnologyreliesonauto-cyclingandDNApolymerasemediatedstranddisplacementDNAsynthesis,amplifyingafewcopiesofDNAto109inlessthananhour.ReversetranscriptioncoupledLAMPcanbeappliedforamplificationofRNAsequences. LAMPemploysfourspeciallydesignedprimers(twoinnerandtwoouter)thatrecognizesixdistinctregionsinthetargetDNA.HybridizationofthefourprimerstothetargetDNAisaverycrucialstepfortheefficiencyofLAMP.Thedesignofthesefourprimersisthereforecriticalforasuccessfulassay. AvoidCrossHomologies LAMPDesignerautomaticallyinterpretsBLASTsearchresultsandavoidsthoseregionstodesignprimersthathavesignificantcrosshomologieswiththedatabase. VerificationBLAST TheprimerscanbeBLASTsearchedagainstadatabasetoverifytheirspecificity. MultiplexLAMPPrimerSet Theprimersdesignedforasequencecanbecheckedformultiplexing.Thefreeenergiesofthemoststablecrosshomologiesbetweentheprimersdesigned,isdisplayedbytheprogram. ExportResult Thedesignedprimersets,alongwiththeirproperties,canbeexportedinstandardcsvorexcelformats. Data&DatabaseManagement Multipleprojectscanbecreated.Dataofmultipleexperimentscanbeeasilymanagedbycreatingaseparateprojectforeachexperiment.Itmaintainsalocaldatabaseforsequenceinformationandsearchresults. TryNow BookaDemo Overview Features Resources DesignPrimersforLoopMediatedIsothermalAmplification LAMPDesignerdesignsefficientprimersforLoop-MediatedIsothermalAmplificationassays,thatamplifyDNAandRNAsequencesatisothermalconditions,eliminatingthenecessityofaPCRsetup.Thetechnologyreliesonauto-cyclingandDNApolymerasemediatedstranddisplacementDNAsynthesis,amplifyingafewcopiesofDNAto109inlessthananhour.ReversetranscriptioncoupledLAMPcanbeappliedforamplificationofRNAsequences. AvoidCrossHomologies LAMPDesignerautomaticallyinterpretsBLASTsearchresultsandavoidsthoseregionstodesignprimersthathavesignificantcrosshomologieswiththedatabase. VerificationBLAST TheprimerscanbeBLASTsearchedagainstadatabasetoverifytheirspecificity. ReadMore>> DesignPrimersforLoopMediatedIsothermalAmplification AvoidCrossHomologies VerificationBLAST MultiplexLAMPPrimerSet ExportResult Data&DatabaseManagement ReadMore>> SystemRequirements HowtoActivate? DownloadTutorial PrintableFlyer NewinthisVersion ScheduleaDemo PriceList OrderNow QUICKLINKS News|Events Testimonials Citations&Reviews Sitemap EXPLORE OurSoftware TryNow FreeEducationalLicense FreeTools ORDERING PriceList GenerateaQuote OrderNow RequestaW-9Form SUPPORT ContactUs BookaMeeting FAQ Suggestions ©1994-PREMIERBiosoft.Allrightsreserved. customerservicesoftwaretechnicalsupport LiveChatbyComm100

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