293T transfection protocol
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- 1Linearization of plasmid DNA before stable transfection - blunt ...
I want to linearize a plasmid and just realized that there is no blunt end restriction site avail...
- 2Preparing Vector DNA For Transfection/Targeting
-Normally targeting vectors need to be linearized before transfection. I prefer to linearize fres...
- 3Stable Transfection - Mirus Bio
When generating a stable cell line, the transfected plasmid undergoes recombination during chromo...
- 4Generation of a stable cell line - Lonza Knowledge Center
Generation of a stable cell line: A positive control plasmid works, ... (Linearized) plasmid shou...
- 5Guidelines for Plasmid DNA Transfection - TW
Stable transfections are more efficient when using linear DNA due to its optimal integration into...